Quantitation, with a new assay, and Theiler's virus capsid protein in the central nervous system of mice

Journal Article · Sat Nov 01 04:00:00 EST 1986 · J. Virol.; (United States)

OSTI ID:6244378

Cash, E; Chamorro, M; Brahic, M

The authors developed a quantitative assay for antigens at the single-cell level. Tissue sections were reacted with (i) a primarily antibody , (ii) a biotinylated secondary antibody, or (iii) /sup 35/S-streptavidin. Binding of streptavidin to cells was quantitated by microscopic autoradiography. They showed that the number of autoradiographic grains was proportional to the amount of antigen per cell. With this assay, they studied the synthesis of Theiler's virus capsid proteins VP1, VP2, and VP3 in permissive BHK cells grown in vitro and in mouse central nervous system (CNS) cells during a persistent infection. They found that synthesis of the three capsid proteins was restricted in mouse CNS cells. Restricted virus replication could play a major role in the persistence of Theiler's virus in mouse CNS cells.

• Research Organization: Institut Pasteur, Paris (France)
• OSTI ID: 6244378
• Journal Information: J. Virol.; (United States), Journal Name: J. Virol.; (United States) Vol. 60:2; ISSN JOVIA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTIGENS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMISTRY
BIOSYNTHESIS
CENTRAL NERVOUS SYSTEM
CHEMISTRY
CYTOCHEMISTRY
DAYS LIVING RADIOISOTOPES
DRUGS
EVEN-ODD NUCLEI
HYBRIDIZATION
ISOTOPES
LIGHT NUCLEI
MAMMALS
MICE
MICROORGANISMS
NERVOUS SYSTEM
NUCLEI
PARASITES
RADIOISOTOPES
RODENTS
SULFUR 35
SULFUR ISOTOPES
SYNTHESIS
VERTEBRATES
VIRUSES