Receptors for Theiler's murine encephalomyelitis virus: characterization by using rabbit antiviral antiserum

Journal Article · Tue Nov 01 04:00:00 EST 1988 · J. Virol.; (United States)

OSTI ID:6868852

Rubio, N; Cuesta, A

An immunological assay was developed to characterize the binding of Theiler's murine encephalomyelitis virus to BHK-21 cell receptors. After absorption of the virus and formaldehyde fixation, rabbit antibodies and Staphylococcus aureus protein A labeled with /sup 125/I formed a specific complex on the surfaces of the cells. The optimal multiplicity of infection in this system was 10 PFU per cell. The virus was internalized at 33 and 37/sup 0/C, but internalization did not take place at 25 or 4/sup 0/C. The binding was proportional to the number of cells and was significant within 30 s. Cell surface receptors were still active after fixation, and only intact viruses were bound, as demonstrated by the lack of binding of the purified, isolated virion proteins VP1, VP2, and VP3.

• Research Organization: Instituo Cajal, Madrid (Spain)
• OSTI ID: 6868852
• Journal Information: J. Virol.; (United States), Journal Name: J. Virol.; (United States) Vol. 62:11; ISSN JOVIA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
CHEMICAL COMPOSITION
DAYS LIVING RADIOISOTOPES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
IMMUNOASSAY
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MAMMALS
MEMBRANE PROTEINS
MICE
MICROORGANISMS
NERVOUS SYSTEM DISEASES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PARASITES
PATHOGENESIS
PROTEINS
RABBITS
RADIOISOTOPES
REACTION KINETICS
RECEPTORS
RODENTS
TRACER TECHNIQUES
VERTEBRATES
VIRUSES