Radioiodination of advenovirus-associated virus external structural proteins. [/sup 125/I]
Journal Article
·
· Virology; (United States)
The three structural polypeptides of adenovirus-associated virus type 3 (AAV-3) were examined to determine their orientation in the viral capsid. Sepharose-bound lactoperoxidase was used to label both dense-band and major-band AAV-3 virions. All three capsid proteins (VP1, VP2, and VP3) were found to be radioiodinated by solid-state lactoperoxidase in both dense-band and major-band virus particles. These findings indicate that the three polypeptides possess sequences that are externally oriented in the virion and that, therefore, no one of the three polypeptides can be considered an exclusively internally core protein.
- Research Organization:
- Brigham Young Univ., Provo, Utah
- OSTI ID:
- 5143520
- Journal Information:
- Virology; (United States), Journal Name: Virology; (United States) Vol. 83:2; ISSN VIRLA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
BETA DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
HALOGENATION
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
INTERMEDIATE MASS NUCLEI
IODINATION
IODINE 125
IODINE ISOTOPES
ISOTOPES
LABELLING
MICROORGANISMS
MOLECULAR STRUCTURE
NUCLEI
ODD-EVEN NUCLEI
ONCOGENIC VIRUSES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXIDOREDUCTASES
PARASITES
PEPTIDES
PEROXIDASES
POLYPEPTIDES
PORPHYRINS
PROTEINS
RADIOISOTOPES
VIRUSES
59 BASIC BIOLOGICAL SCIENCES
BETA DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
HALOGENATION
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
INTERMEDIATE MASS NUCLEI
IODINATION
IODINE 125
IODINE ISOTOPES
ISOTOPES
LABELLING
MICROORGANISMS
MOLECULAR STRUCTURE
NUCLEI
ODD-EVEN NUCLEI
ONCOGENIC VIRUSES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXIDOREDUCTASES
PARASITES
PEPTIDES
PEROXIDASES
POLYPEPTIDES
PORPHYRINS
PROTEINS
RADIOISOTOPES
VIRUSES