Immobilized purified folate-binding protein: binding characteristics and use for quantifying folate in erythrocytes

Journal Article · Sat Aug 01 04:00:00 EDT 1987 · Clin. Chem. (Winston-Salem, N.C.); (United States)

OSTI ID:6025342

Hansen, S I; Holm, J; Nexo, E

Purified folate-binding protein from cow's milk was immobilized on monodisperse polymer particles (Dynospheres) activated by rho-toluenesulfonyl chloride. Leakage from the spheres was less than 0.1%, and the binding properties were similar to those of the soluble protein with regard to dissociation, pH optimum for binding pteroylglutamic acid, and specificity for binding various folate derivatives. We used the immobilized folate-binding protein as binding protein in an isotope-dilution assay for quantifying folate in erythrocytes. The detection limit was 50 nmol/L and the CV over a six-month period was 2.3% (means = 1.25 mumol/L, n = 15). The reference interval, for folate measured in erythrocytes of 43 blood donors, was 0.4-1.5 mumol/L.

• Research Organization: Central Hospital, Hillerod, Denmark
• OSTI ID: 6025342
• Journal Information: Clin. Chem. (Winston-Salem, N.C.); (United States), Journal Name: Clin. Chem. (Winston-Salem, N.C.); (United States) Vol. 33:8; ISSN CLCHA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMALS
AROMATICS
AZAARENES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOXYLIC ACIDS
CATTLE
CHEMICAL PROPERTIES
DOMESTIC ANIMALS
DRUGS
ERYTHROCYTES
FOLIC ACID
FOOD
HEMATINICS
HEMATOLOGIC AGENTS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPE DILUTION
KINETICS
MAMMALS
MATERIALS
MILK
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
POLYMERS
PROTEINS
PTERIDINES
REACTION KINETICS
RUMINANTS
SPECIFICITY
TRACER TECHNIQUES
VERTEBRATES
VITAMIN B GROUP
VITAMINS