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Detergent activation of the binding protein in the folate radioassay

Journal Article · · Clin. Chem. (Winston-Salem, N.C.); (United States)
OSTI ID:5761057
; ;
A minor cow's whey protein associated with ..beta..-lactoglobulin is used as binding protein in the competitive radioassay for serum and erythrocyte folate. Seeking to optimize the assay, we tested the performance of binder solutions of increasing purity. The folate binding protein was isolated from cow's whey by means of CM-Sepharose CL-6B cation-exchange chromatography, and further purified on a methotrexate-AH-Sepharose 4B affinity matrix. In contrast to ..beta..-lactoglobulin, the purified protein did not bind folate unless the detergents cetyltrimethylammonium (10 mmol/Ll) or Triton X-100 (1 g/L) were present. Such detergent activation was not needed in the presence of serum. There seems to be a striking analogy between these phenomena and the well-known reactivation of certain purified membrane-derived enzymes by surfactants (lipids/detergents).
OSTI ID:
5761057
Journal Information:
Clin. Chem. (Winston-Salem, N.C.); (United States), Journal Name: Clin. Chem. (Winston-Salem, N.C.); (United States) Vol. 28:1; ISSN CLCHA
Country of Publication:
United States
Language:
English

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Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ADDITIVES
AMINO ACIDS
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD SERUM
BODY FLUIDS
CARBOXYLIC ACIDS
CATTLE
CHEMICAL ACTIVATION
COWS
DATA
DETERGENTS
DOMESTIC ANIMALS
DRUGS
EMULSIFIERS
ERYTHROCYTES
EXPERIMENTAL DATA
FOLIC ACID
FOOD
HEMATINICS
HEMATOLOGIC AGENTS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
INFORMATION
LABELLED COMPOUNDS
LIGANDS
MAMMALS
MATERIALS
MILK
NUMERICAL DATA
OPTIMIZATION
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PROTEINS
PTERIDINES
RADIOASSAY
RESPONSE MODIFYING FACTORS
RUMINANTS
SURFACTANTS
TRITIUM COMPOUNDS
UPTAKE
VERTEBRATES
VITAMIN B GROUP
VITAMINS
WETTING AGENTS