Evidence that the low-affinity folate-binding protein in erythrocyte hemolysate is identical to hemoglobin

Journal Article · Wed Jul 01 00:00:00 EDT 1981 · Clin. Chem. (Winston-Salem, N.C.); (United States)

OSTI ID:5707773

Hansen, S I; Holm, J; Lyngbye, J

Gel filtration studies on erythrocyte hemolysate demonstrated the presence of a folate binding protein, apparently of the low-affinity type, that co-elutes with hemoglobin. Further, the folate binder eluted with a low salt concentration after DEAE-Sepharose CL-6B anion-exchange chromatography of erythrocyte hemolysate at pH 6.3. The chromatographic behavior of hemoglobin labeled with (3H)folate was so similar to that of the present binder as to suggest that the folate binder in erythrocytes is in fact hemoglobin.

• OSTI ID: 5707773
• Journal Information: Clin. Chem. (Winston-Salem, N.C.); (United States), Journal Name: Clin. Chem. (Winston-Salem, N.C.); (United States) Vol. 27:7; ISSN CLCHA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AFFINITY
AMINO ACIDS
BIOCHEMICAL REACTION KINETICS
BIOCHEMISTRY
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOXYLIC ACIDS
CHEMISTRY
DRUGS
ERYTHROCYTES
FOLIC ACID
GLOBIN
HEMATINICS
HEMATOLOGIC AGENTS
HEMOGLOBIN
HEMOLYSIS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LYSIS
MATERIALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PATHOLOGICAL CHANGES
PH VALUE
PIGMENTS
PORPHYRINS
PROTEINS
PTERIDINES
REACTION KINETICS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VITAMIN B GROUP
VITAMINS