Some nutritional effects of folate-binding protein in bovine milk on the bioavailability of folate to rats
The excretions of folate compounds into both the urine and bile were investigated in rats after the administration of pteroylglutamic acid (PteGlu) with or without the folate-binding protein (FBP) prepared from bovine milk. When the sample solution, containing either free or bound (/sup 3/H)PteGlu (i.e., bound to the FBP from milk), was delivered to rats intragastrically via oral intubation, the amounts of (/sup 3/H)PteGlu excreted into the feces did not change. On the other hand, the urinary excretion of /sup 3/H-labeled folate compounds, especially (/sup 3/H)5-methyltetrahydrofolic acid (5-CH/sub 3/-H/sub 4/PteGlu), after the administration of bound (/sup 3/H)PteGlu was significantly lower (P less than 0.01) than that after the administration of free (/sup 3/H)PteGlu. The urinary excretion of (/sup 3/H)5-CH/sub 3/-H/sub 4/PteGlu was directly proportional to the initial amount of free (/sup 3/H)PteGlu administered. The similar effect of FBP was also observed when the biliary excretion of /sup 3/H-labeled folate compounds was investigated in situ. Furthermore, the incorporation of (/sup 3/H)PteGlu into folate-requiring intestinal microorganisms was considerably reduced when it was bound to FBP. These results suggest that milk FBP has some nutritional effects on the bioavailability of folate in vivo.
- Research Organization:
- Department of Food Science and Technology, Faculty of Agriculture, Kyoto University, Japan
- OSTI ID:
- 6728641
- Journal Information:
- J. Nutr.; (United States), Vol. 114:4
- Country of Publication:
- United States
- Language:
- English
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FOLIC ACID
EXCRETION
METABOLISM
CARBON 14 COMPOUNDS
DIET
LIQUID COLUMN CHROMATOGRAPHY
MILK
RATS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
AMINO ACIDS
ANIMALS
AROMATICS
AZAARENES
BIOLOGICAL MATERIALS
BODY FLUIDS
CARBOXYLIC ACIDS
CHROMATOGRAPHY
CLEARANCE
DRUGS
FOOD
HEMATINICS
HEMATOLOGIC AGENTS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MATERIALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PTERIDINES
RODENTS
SEPARATION PROCESSES
VERTEBRATES
VITAMIN B GROUP
VITAMINS
550501* - Metabolism- Tracer Techniques