Proliferative kinetics of human lymphocytes in culture measured by autoradiography and sister chromatid differential staining
Journal Article
·
· Exp. Cell Res.; (United States)
A simple combination of autoradiography, to determine when a cell synthesized DNA, and sister chromatid differential staining, to determine how many times a cell has divided, was used to follow up the proliferating fate of human lymphocytes in culture. Cells were incubated continuously with 5-bromodeoxyuridine (BrdU) and pulse-labelled with 0.1 ..mu..Ci/ml (/sup 3/H)thymidine at various times after stimulation with phytohemagglutinin (PHA). The cells were then harvested at 4 h intervals up to 72 h, and the percentage of labelled mitoses was determined separately in first, second, or third division cells. The data showed that the cycling cells, whether they began cycling at earlier or later times after stimulation, had about the same generation times of 12-14 h. This confirms that the heterogeneity of cell generations seen in short-term lymphocyte cultures is in large part due to the difference in the times when cells began cell cycling in response to PHA. 34 references, 2 figures, 1 table.
- Research Organization:
- Univ. of California, San Francisco
- OSTI ID:
- 5997976
- Journal Information:
- Exp. Cell Res.; (United States), Journal Name: Exp. Cell Res.; (United States) Vol. 145; ISSN ECREA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Cytokinetic analysis of the impaired proliferative response of peripheral lymphocytes from aged humans to phytohemagglutinin. [BUDR]
Variation in the baseline sister chromatid exchange frequency in human lymphocytes
Sister chromatid exchanges in human lymphocytes exposed to ionizing radiation during G/sub 0/. [. gamma. rays]
Journal Article
·
Tue May 01 00:00:00 EDT 1979
· J. Exp. Med.; (United States)
·
OSTI ID:6868532
Variation in the baseline sister chromatid exchange frequency in human lymphocytes
Journal Article
·
Mon Dec 31 23:00:00 EST 1979
· Environ. Mutagen.; (United States)
·
OSTI ID:6502895
Sister chromatid exchanges in human lymphocytes exposed to ionizing radiation during G/sub 0/. [. gamma. rays]
Journal Article
·
Fri Jun 01 00:00:00 EDT 1979
· Radiat. Res.; (United States)
·
OSTI ID:5993950
Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AGGLUTININS
ANIMAL CELLS
ANTIBODIES
AUTORADIOGRAPHY
AZINES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CELL CYCLE
CELL PROLIFERATION
CHROMOSOMAL ABERRATIONS
CONNECTIVE TISSUE CELLS
DATA
DNA
EXPERIMENTAL DATA
HEMAGGLUTININS
HETEROCYCLIC COMPOUNDS
INFORMATION
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LEUKOCYTES
LYMPHOCYTES
MATERIALS
MITOGENS
MUCOPROTEINS
MUTATIONS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHYTOHEMAGGLUTININ
POLYSACCHARIDES
PROTEINS
PYRIMIDINES
REACTION KINETICS
RIBOSIDES
SACCHARIDES
SISTER CHROMATID EXCHANGES
SOMATIC CELLS
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
59 BASIC BIOLOGICAL SCIENCES
AGGLUTININS
ANIMAL CELLS
ANTIBODIES
AUTORADIOGRAPHY
AZINES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CELL CYCLE
CELL PROLIFERATION
CHROMOSOMAL ABERRATIONS
CONNECTIVE TISSUE CELLS
DATA
DNA
EXPERIMENTAL DATA
HEMAGGLUTININS
HETEROCYCLIC COMPOUNDS
INFORMATION
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LEUKOCYTES
LYMPHOCYTES
MATERIALS
MITOGENS
MUCOPROTEINS
MUTATIONS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHYTOHEMAGGLUTININ
POLYSACCHARIDES
PROTEINS
PYRIMIDINES
REACTION KINETICS
RIBOSIDES
SACCHARIDES
SISTER CHROMATID EXCHANGES
SOMATIC CELLS
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS