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Variation in the baseline sister chromatid exchange frequency in human lymphocytes

Journal Article · · Environ. Mutagen.; (United States)
; ; ;
The utility of the sister chromatid exchange (SCE) assay for human population studies is potentially limited by the variability associated with individual baseline SCE frequencies. This investigation identifies and quantifies the major sources of preparative and biological variation associated with the determination of baseline SCE frequencies in cultured human lymphocytes. Much of the variation in lymphocyte SCE frequencies is attributable to the amount of bromodeoxyuridine (BrdUrd) available per lymphocyte. Other variations in the baseline frequency result from culture-to-culture and slide-to-slide differences. The effect of cell-to-cell differences in baseline SCE frequency among donors can be minimized by increasing the number of cells scored per donor. These results indicate the feasibility of applying the SCE bioassay to humans as a measure of environmental stress.
Research Organization:
Lawrence Livermore Lab., CA
DOE Contract Number:
W-7405-ENG-48
OSTI ID:
6502895
Journal Information:
Environ. Mutagen.; (United States), Journal Name: Environ. Mutagen.; (United States) Vol. 2; ISSN ENMUD
Country of Publication:
United States
Language:
English

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Related Subjects

550300 -- Cytology
550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
ANTIMETABOLITES
AZINES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
BROMOURACILS
BUDR
CHROMOSOMAL ABERRATIONS
CONNECTIVE TISSUE CELLS
DRUGS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MAN
MATERIALS
MATHEMATICS
MUTATIONS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC BROMINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PRIMATES
PYRIMIDINES
RIBOSIDES
SISTER CHROMATID EXCHANGES
SOMATIC CELLS
STATISTICS
URACILS
VERTEBRATES