Cytokinetic analysis of the impaired proliferative response of peripheral lymphocytes from aged humans to phytohemagglutinin. [BUDR]
Journal Article
·
· J. Exp. Med.; (United States)
The effect of donor age on the rate of cell entry into the proliferating pool and subsequent cell cycle duration for peripheral lymphocytes stimulated by phytohemagglutinin (PHA) were examined by using the bromodeoxyuridine incorporation-differential staining technique. Distribution curves for the appearance of metaphase cells in successive generations as a function of culture time were obtained and analyzed both graphically and by a computer simulation model. Peripheral lymphocytes from aged individuals ( approx. 75 yr) were stimulated by PHA at approximately one-half of the rate of peripheral lymphocytes from young individuals ( approx. 21 yr). Subsequent cell-cycle durations were estimated to range from 10.0 to 25.0 h for aged individual lymphocyte cultures and 10.6 to 15.6 h for young individual lymphocyte cultures. The possible significance of these findings to aging in general is discussed.
- Research Organization:
- Brookhaven National Lab., Upton, NY
- OSTI ID:
- 6868532
- Journal Information:
- J. Exp. Med.; (United States), Journal Name: J. Exp. Med.; (United States) Vol. 00; ISSN JEMEA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Proliferative kinetics of human lymphocytes in culture measured by autoradiography and sister chromatid differential staining
Monitoring of chimerism using fluorescence in situ hybridization in a child with severe combined immune deficiency following bone marrow transplant
Spontaneous and mitogen-induced proliferative activity of mononuclear cells in patients with pollinosis
Journal Article
·
Fri Dec 31 23:00:00 EST 1982
· Exp. Cell Res.; (United States)
·
OSTI ID:5997976
Monitoring of chimerism using fluorescence in situ hybridization in a child with severe combined immune deficiency following bone marrow transplant
Journal Article
·
Thu Sep 01 00:00:00 EDT 1994
· American Journal of Human Genetics
·
OSTI ID:134618
Spontaneous and mitogen-induced proliferative activity of mononuclear cells in patients with pollinosis
Journal Article
·
Sat Jun 01 00:00:00 EDT 1985
· Bull. Exp. Biol. Med. (Engl. Transl.); (United States)
·
OSTI ID:6934995
Related Subjects
550300* -- Cytology
59 BASIC BIOLOGICAL SCIENCES
AGE DEPENDENCE
AGGLUTININS
ANIMAL CELLS
ANIMALS
ANTIMETABOLITES
ANTIMITOTIC DRUGS
AZINES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
BROMOURACILS
BUDR
CARBOHYDRATES
CELL DIVISION
CELL PROLIFERATION
CONNECTIVE TISSUE CELLS
CYTOLOGICAL TECHNIQUES
DRUGS
HEMAGGLUTININS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
IN VITRO
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MAN
MATERIALS
MITOGENS
MITOSIS
MUCOPROTEINS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC BROMINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHYTOHEMAGGLUTININ
POLYSACCHARIDES
PRIMATES
PROTEINS
PYRIMIDINES
RIBOSIDES
SACCHARIDES
SOMATIC CELLS
URACILS
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
AGE DEPENDENCE
AGGLUTININS
ANIMAL CELLS
ANIMALS
ANTIMETABOLITES
ANTIMITOTIC DRUGS
AZINES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
BROMOURACILS
BUDR
CARBOHYDRATES
CELL DIVISION
CELL PROLIFERATION
CONNECTIVE TISSUE CELLS
CYTOLOGICAL TECHNIQUES
DRUGS
HEMAGGLUTININS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
IN VITRO
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MAN
MATERIALS
MITOGENS
MITOSIS
MUCOPROTEINS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC BROMINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHYTOHEMAGGLUTININ
POLYSACCHARIDES
PRIMATES
PROTEINS
PYRIMIDINES
RIBOSIDES
SACCHARIDES
SOMATIC CELLS
URACILS
VERTEBRATES