Role of in vitro factors in ozone toxicity for cultured rat lung fibroblasts

Journal Article · Fri Jan 01 04:00:00 EST 1982 · Drug Chem. Toxicol.; (United States)

DOI:https://doi.org/10.3109/01480548209041053· OSTI ID:5991569

Wenzel, D G; Morgan, D L

Ozone toxicity for cultured rat lung fibroblasts was concentration dependent and was affected by the manner in which ozone was delivered to the cells, i.e. cultures were either rotated with a thin moving overlay of medium or were stationary with a fixed layer of medium between the cells and the gas phase. The influence of culture medium components and culture dish composition on the toxicity of ozone were also investigated. Cell viability, used to measure ozone toxicity, was quantified by the chromium-51 release assay, and by a viability index calculated from the percentage of cells stained with a vital dye combined with the decrease in cell number as determined by DNA measurements. During stationary ozone exposure, toxicity appeared to be mediated primarily by hydrogen peroxide and could be inhibited by catalase or fetal bovine serum when measured by the viability index. During rotated exposure, catalase and fetal bovine serum provided no protection when measured by the viability index, however, when measured by the chromium-51 release assay, fetal bovine serum was partially protective. The effect of ozone on the fibroblasts was not influenced by whether culture dishes were glass or plastic, or whether the culture medium was balanced salt solution or complete chemically-defined medium.

• Research Organization: Department of Pharmacology and Toxicology, School of Pharmacy, University of Kansas, Lawrence
• OSTI ID: 5991569
• Journal Information: Drug Chem. Toxicol.; (United States), Journal Name: Drug Chem. Toxicol.; (United States) Vol. 5:3; ISSN DCTOD
• Country of Publication: United States
• Language: English

Related Subjects

550301 -- Cytology-- Tracer Techniques
560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BODY
CALCULATION METHODS
CELL CULTURES
CHEMICAL ANALYSIS
CHROMIUM 51
CHROMIUM ISOTOPES
CONNECTIVE TISSUE CELLS
DNA
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
FIBROBLASTS
IN VITRO
INTERMEDIATE MASS NUCLEI
ISOTOPES
LABELLED COMPOUNDS
LUNGS
MAMMALS
METABOLISM
NUCLEI
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
OZONE
QUANTITATIVE CHEMICAL ANALYSIS
RADIOISOTOPES
RATS
RESPIRATORY SYSTEM
RODENTS
SENSITIVITY
SOMATIC CELLS
TOXICITY
VERTEBRATES
VIABILITY