In vitro system for exposure of lung cells to gases: effects of ozone on rate macrophages

Journal Article · Tue Jan 01 00:00:00 EST 1985 · J. Toxicol. Environ. Health; (United States)

DOI:https://doi.org/10.1080/15287398509530723· OSTI ID:6254005

Valentine, R

A test system was developed for the in vitro exposure of lung cells to gases. The exposure system was used to evaluate ozone (O3) injury to pulmonary alveolar macrophages (PAM) obtained from Spague-Dawley rats by bronchopulmonary lavage. Ozone exposures were conducted within temperature-controlled stainless-steel and Plexiglas chambers that contained glass petri dishes affixed to a revolving, inclined platform. Cell exposures were accomplished by rotation of the platform at 1 rpm to alternately expose PAM monolayers to culture media and O3. The system provided stable, O3-containing atmospheres and permitted simultaneous in vitro exposures at three O3 concentrations. In vitro exposure of PAM monolayers for 2 h at chamber concentrations ranging from 0.2 to 6.1 ppm O3 was associated with a significant, concentration-related reduction of latex-bead phagocytosis in rotated PAM cultures. In contrast, PAM that were similarly exposed in nonrotated dishes placed horizontally and covered with a stationary layer of media (1.5 mm depth) were not affected. Other parameters of cell function, including PAM viability and adherence, were unchanged compared to unexposed or horizontal, nonrotated controls. The inability to observe adverse effects among the nonrotated cultures is consistent with the impaired diffusion of O3 through the comparatively thick media overlay in stationary cultures. The in vitro system provides a realistic simulation of lung cell exposure to O3 and represents a useful model to study the toxicity of gases on cultured cells.

• Research Organization: New York Univ., New York
• OSTI ID: 6254005
• Journal Information: J. Toxicol. Environ. Health; (United States), Vol. 16:1
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
LUNGS
BIOLOGICAL MODELS
MACROPHAGES
PHAGOCYTOSIS
OZONE
TOXICITY
DOSE-RESPONSE RELATIONSHIPS
EXPOSURE CHAMBERS
IN VITRO
RATS
RESPONSE MODIFYING FACTORS
VIABILITY
ANIMAL CELLS
ANIMALS
BODY
CONNECTIVE TISSUE CELLS
MAMMALS
ORGANS
PHAGOCYTES
RESPIRATORY SYSTEM
RODENTS
SOMATIC CELLS
VERTEBRATES
560301* - Chemicals Metabolism & Toxicology- Cells- (-1987)