Rat pulmonary alveolar macrophages in-vitro cytotoxicity to six metal dusts. Technical report, May-August 1985
Pulmonary alveolar macrophages (PAMs) were harvested from rats by bronchopulmonary lavage and exposed in vitro to aqueous suspensions of six metal dusts: aluminum, silicated aluminum, carbon, graphite, titanium dioxide, and silica. The LC50 value for each dust was calculated by logarithmic linear regression analysis of the toxicity data from the experimental exposure concentrations. Cytotoxicity was determined by metal concentration-dependent inability of PAMs to exclude the trypan blue dye from their cytroplasm. After exposure, PAMs usually bore numerous bound metal particles; but phagocytosis was rare, probably because the cells were not activated in situ prior to removal from the lungs.
- Research Organization:
- Chemical Research, Development and Engineering Center, Aberdeen Proving Ground, MD (USA)
- OSTI ID:
- 5234424
- Report Number(s):
- AD-A-189040/9/XAB; CRDEC-TR-88037
- Country of Publication:
- United States
- Language:
- English
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54 ENVIRONMENTAL SCIENCES
550900* -- Pathology
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMAL TISSUES
BODY
CARBON
CHALCOGENIDES
CONNECTIVE TISSUE CELLS
DOCUMENT TYPES
DUSTS
ELEMENTAL MINERALS
ELEMENTS
GRAPHITE
MACROPHAGES
METALS
MINERALS
NONMETALS
OXIDE MINERALS
OXIDES
OXYGEN COMPOUNDS
PARTICLES
PARTICULATES
PHAGOCYTES
PHAGOCYTOSIS
PROGRESS REPORT
RETICULOENDOTHELIAL SYSTEM
SILICA
SILICATES
SILICON COMPOUNDS
SILICON OXIDES
SOMATIC CELLS
TISSUES
TOXICITY