Ozone-generating system and chamber for testing injury to cultured cells

Journal Article · · Arch. Environ. Health; (United States)

DOI:https://doi.org/10.1080/00039896.1979.10667449· OSTI ID:5973461

Wenzel, D G; Kotek, J J; Morgan, D L; Wrobel, W J

A system was constructed which alternately exposed cultured cells to specific concentrations of ozone in the gas phase and then to cell culture medium. It was designed to produce a constant mass transfer between the gas phase and the exposed cell phase. Monolayers of neonatal rat lung fibroblasts cultured in miniature glass dishes were used to test for ozone toxicity. The cells were alternately exposed to ozone (0.05, 0.15, 0.45, 1.35, and 4.05 ppm) for 30 sec and then to culture medium for 30 sec over a 1-hr period. Toxicity was measured as inhibition of cell growth 4 days after exposure to ozone. Growth was significantly inhibited at all concentrations with a 50% inhibitory concentration of 0.8 ppm. The system described was effective for quantifying ozone toxicity for cultured lung cells. 29 references, 5 figures.

• Research Organization: Univ. of Kansas, Lawrence
• OSTI ID: 5973461
• Journal Information: Arch. Environ. Health; (United States), Journal Name: Arch. Environ. Health; (United States) Vol. 34:6; ISSN AEHLA
• Country of Publication: United States
• Language: English

Related Subjects

560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AIR POLLUTION
BIOASSAY
BIOLOGICAL EFFECTS
CELL CULTURES
COMPARATIVE EVALUATIONS
DESIGN
EQUIPMENT
FABRICATION
INHIBITION
INJURIES
LABORATORY EQUIPMENT
MASS TRANSFER
OZONE
PERFORMANCE TESTING
POLLUTION
TESTING