Reduced growth factor requirement of keloid-derived fibroblasts may account for tumor growth

Journal Article · Fri Jan 01 04:00:00 EST 1988 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.85.2.587· OSTI ID:6142630

Russell, S B; Trupin, K M; Rodriguez-Eaton, S; Russell, J D; Trupin, J S

Keloids are benign dermal tumors that form during an abnormal wound-healing process is genetically susceptible individuals. Although growth of normal and keloid cells did not differ in medium containing 10% (vol/vol) fetal bovine serum, keloid culture grew to significantly higher densities than normal cells in medium containing 5% (vol/vol) fetal bovine serum, keloid cultures grew to significantly higher densities than normal cells in medium containing 5% (vol/vol) plasma or 1% fetal bovine serum. Conditioned medium from keloid cultures did not stimulate growth of normal cells in plasma nor did it contain detectable platelet-derived growth factor or epidermal growth factor. Keloid fibroblasts responded differently than normal adult fibroblasts to transforming growth factor ..beta... Whereas transforming growth factor ..beta.. reduced growth stimulation by epidermal growth factor in cells from normal adult skin or scars, it enhanced the activity of epidermal growth factor in cells from normal adult skin or scars, it enhanced the activity of epidermal growth factor in cells from keloids. Normal and keloid fibroblasts also responded differently to hydrocortisone: growth was stimulated in normal adult cells and unaffected or inhibited in keloid cells. Fetal fibroblasts resembled keloid cells in their ability to grow in plasma and in their response to hydrocortisone. The ability of keloid fibroblasts to grow to higher cell densities in low-serum medium than cells from normal adult skin or from normal early or mature scars suggests that a reduced dependence on serum growth factors may account for their prolonged growth in vivo. Similarities between keloid and fetal cells suggest that keloids may result from the untimely expression of growth-control mechanism that is developmentally regulated.

• Research Organization: Meharry Medical College, Nashville, TN (USA)
• OSTI ID: 6142630
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 85:2; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADRENAL HORMONES
ANIMAL CELLS
AZINES
BIOCHEMISTRY
BIOLOGICAL RECOVERY
BLOOD SERUM
BODY
CELL PROLIFERATION
CHEMISTRY
CONNECTIVE TISSUE CELLS
CORTICOSTEROIDS
CYTOLOGICAL TECHNIQUES
DISEASES
DOSE-RESPONSE RELATIONSHIPS
FIBROBLASTS
GLUCOCORTICOIDS
GROWTH FACTORS
HEALING
HETEROCYCLIC COMPOUNDS
HYDROCORTISONE
HYDROXY COMPOUNDS
INJURIES
KETONES
LABELLED COMPOUNDS
MITOGENS
NEOPLASMS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PATHOLOGICAL CHANGES
PREGNANES
PROTEINS
PYRIMIDINES
RECOVERY
RIBOSIDES
SKIN
SOMATIC CELLS
STEROIDS
THYMIDINE
TRITIUM COMPOUNDS
WOUNDS