Modulation of. beta. -adrenergic response in rat brain astrocytes by serum and hormones

Journal Article · Tue Jan 01 04:00:00 EST 1985 · J. Cell. Physiol.; (United States)

DOI:https://doi.org/10.1002/jcp.1041220112· OSTI ID:5710137

Wu, D K; Morrison, R S; de Vellis, J

Purified astrocyte cultures from neonatal rat cerebrum respond to isoproterenol, a ..beta..-adrenergic agonist, with a transient rise in cAMP production. This astroglial property was regulated by serum, a chemically defined medium (serum-free medium plus hydrocortisone, putrescine, prostaglandin F/sub 2/, insulin, and fibroblast growth factor) and epidermal growth factor. Compared to astrocytes grown in serum-supplemented medium, astrocytes grown in the chemically defined medium were nonresponsive to isoproterenol stimulation, and this difference did not appear to be due to selection of a subpopulation of cells by either medium. The data suggest that a decreased ..beta..-adrenergic receptor number and an increased degradation of cAMP may account for the reduced response to ..beta..-adrenergic stimulation. The nonresponsive state of astrocytes in the defined medium was reversible when the medium was replaced with serum-supplemented medium. An active substance(s) in serum was responsible for restoring the responsiveness of astrocytes. Each of the five components of the defined medium had little effect by itself; however, together they acted synergistically to desensitize astrocytes to ..beta..-adrenergic stimulation. On the other hand, epidermal growth factor, a potent mitogen for astrocytes, was very competent by itself in reducing the cAMP response of astrocytes to ..beta..-adrenergic stimulation. Thus purified astrocytes grown in the chemically defined medium appear to be a good model for the study of hormonal interactions and of serum factors which may modulate the ..beta..-adrenergic response.

• Research Organization: Univ. of California, Los Angeles
• DOE Contract Number: AC03-76SF00012
• OSTI ID: 5710137
• Journal Information: J. Cell. Physiol.; (United States), Journal Name: J. Cell. Physiol.; (United States) Vol. 122:1; ISSN JCLLA
• Country of Publication: United States
• Language: English

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550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ADRENAL HORMONES
AMINES
AMP
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BIOCHEMISTRY
BIOLOGICAL MODELS
BODY
BRAIN
CELL CULTURES
CENTRAL NERVOUS SYSTEM
CHEMISTRY
CONNECTIVE TISSUE CELLS
CORTICOSTEROIDS
CULTURE MEDIA
DRUGS
EPIDERMIS
EPITHELIUM
FIBROBLASTS
GLUCOCORTICOIDS
GROWTH
HORMONES
HYDROCORTISONE
HYDROXY COMPOUNDS
INSULIN
KETONES
MAMMALS
NERVOUS SYSTEM
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
PREGNANES
PROSTAGLANDINS
PUTRESCINE
RATS
RECEPTORS
RODENTS
SKIN
SOMATIC CELLS
STEROID HORMONES
STEROIDS
SYMPATHOMIMETICS
SYNERGISM
TISSUES
VERTEBRATES