Growth of purified astrocytes in a chemically defined medium

Journal Article · Sun Nov 01 04:00:00 EST 1981 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.78.11.7205· OSTI ID:5858574

Morrison, R S; De Vellis, J

Astrocytes purified from primary cultures of neonatal rat cerebrum can not be grown in a synthetic medium supplemented with putrescine, prostaglandin F/sub 2//sub ..cap alpha../, insulin, fibroblast growth factor, and hydrocortisone. These five supplements have a marked synergistic effect on growth when used in combination but have little effect when used individually. Astrocytes grown in the defined medium exhibit dramatic changes in morphological characteristics in comparison to cells grown in serum-free or serum-supplemented medium. In addition, these cells express the astrocyte-specific marker glial fibrillary acidic protein and are estimated by several criteria to be greater than 95% astrocytes.

• Research Organization: Univ. of California, Los Angeles
• OSTI ID: 5858574
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 78:11; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550300* -- Cytology
59 BASIC BIOLOGICAL SCIENCES
ADRENAL HORMONES
AMINES
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BODY
BRAIN
CENTRAL NERVOUS SYSTEM
CEREBRUM
CONNECTIVE TISSUE CELLS
CORTICOSTEROIDS
FIBROBLASTS
GLUCOCORTICOIDS
GROWTH
HORMONES
HYDROCORTISONE
HYDROXY COMPOUNDS
INSULIN
KETONES
MAMMALS
MORPHOLOGICAL CHANGES
NEONATES
NERVE CELLS
NERVOUS SYSTEM
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
PREGNANES
PROSTAGLANDINS
PUTRESCINE
RATS
RODENTS
SOMATIC CELLS
STEROID HORMONES
STEROIDS
SYNERGISM
VERTEBRATES