Effect of continuous, whole-body gamma irradiation upon canine lymphohematopoietic (CFU-GM, CFU-L) progenitors and a possible hematopoietic regulatory population

Journal Article · Fri Feb 01 04:00:00 EST 1985 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3576399· OSTI ID:5978114

Klein, A K; Dyck, J A; Shimizu, J A; Stitzel, K A; Wilson, F D; Cain, G R

Clonogenic assays for granulocytes-macrophages (CFU-GM) in bone marrow and for T lymphocytes (CFU-L) in peripheral blood were performed on dogs continuously exposed to /sup 60/Co irradiation (0.02, 0.04, or 0.11 Gy/day). When decreased numbers of CFU-GM were observed they correlated well with the clinical status of the dogs but were not generally associated with increasing cumulative doses of absorbed irradiation. In clinically normal, irradiated animals, decreased CFU-GM values and myeloid-erythroid ratios were observed, suggesting that chronic irradiation may affect the granulocytic series well before decreased peripheral blood values are seen. In hypocellular dogs the number of CFU-GM were significantly decreased compared to values obtained from control or clinically normal irradiated dogs, while virtually no CFU-GM were observed in the leukemic dogs. Proliferative capacity of T lymphocytes (CFU-L) was not affected by either increasing absorbed irradation or the presence of leukemia, D/sub 0/ values were determined on marrow fibroblastic cells to ascertain whether a radioresistant subpopulation of stromal elements would result from continous in vivo irradiation. These radioresistant marrow fibroblastic cells were assayed for their ability to support normal granulopoiesis and found to be not significantly different from control fibroblasts.

• Research Organization: Univ. of California, Davis
• DOE Contract Number: AC03-76SF00472
• OSTI ID: 5978114
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 101:2; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BEAGLES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CELL PROLIFERATION
CFU
CHRONIC EXPOSURE
CHRONIC IRRADIATION
COBALT 60
COBALT ISOTOPES
CONNECTIVE TISSUE CELLS
DATA
DISEASES
DOGS
ELECTROMAGNETIC RADIATION
EXPERIMENTAL DATA
EXTERNAL IRRADIATION
FIBROBLASTS
GAMMA RADIATION
HEMATOPOIETIC SYSTEM
HEMIC DISEASES
INFORMATION
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IONIZING RADIATIONS
IRRADIATION
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LEUKEMIA
LEUKOCYTES
LYMPHOCYTES
MACROPHAGES
MAMMALS
MATERIALS
MINUTES LIVING RADIOISOTOPES
NEOPLASMS
NUCLEI
NUMERICAL DATA
ODD-ODD NUCLEI
PHAGOCYTES
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RADIOISOTOPES
SOMATIC CELLS
VERTEBRATES
WHOLE-BODY IRRADIATION
YEARS LIVING RADIOISOTOPES