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Technetium-99m labeling of polymorphonuclear leukocytes: preparation with two different stannous agents

Journal Article · · J. Nucl. Med.; (United States)
OSTI ID:5970321
;
A technique for in vitro labeling of human polymorphonuclear leukocytes with Tc-99m is described. Titration of stannous fluoride and stannous pyrophosphate concentrations for pretinning was performed, and optimal amounts of the stannous agents were added to polymorphonuclear leukocytes efficiency isolated from 100 ml of blood. Labeling with 10-15 mCi Tc-99m resulted, after three washings of cell suspensions, in yield of 1.6-4.8 mCi, corresponding to 20.5-33.5% of added tracer. Cell-bound activity in the final cell suspensions was 92.3% +/- 1.9 of the added dose. Cell function was not impaired by the labeling technique. Sterility and exclusion of bacterial endotoxins in the final cell suspensions were demonstrated. The method may prove of diagnostic value in the isolation, labeling, and reinjection of autologous leukocytes for scintigraphic imaging of acute inflammatory lesions.
Research Organization:
Herlev Univ. Hospital, Denmark
OSTI ID:
5970321
Journal Information:
J. Nucl. Med.; (United States), Journal Name: J. Nucl. Med.; (United States) Vol. 26:1; ISSN JNMEA
Country of Publication:
United States
Language:
English

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Related Subjects

38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY
400702* -- Radiochemistry & Nuclear Chemistry-- Properties of Radioactive Materials
560122 -- Radiation Effects on Cells-- Internal Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BLOOD
BLOOD CELLS
BODY FLUIDS
CHEMICAL PREPARATION
DRUGS
FLUORIDES
FLUORINE COMPOUNDS
HALIDES
HALOGEN COMPOUNDS
HOURS LIVING RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
ISOMERIC NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LABELLED COMPOUNDS
LABELLING
LEUKOCYTES
MATERIALS
NUCLEI
ODD-EVEN NUCLEI
OXYGEN COMPOUNDS
PHOSPHATES
PHOSPHORUS COMPOUNDS
RADIATION EFFECTS
RADIOISOTOPES
RADIOPHARMACEUTICALS
SYNTHESIS
TECHNETIUM 99
TECHNETIUM ISOTOPES
TIN COMPOUNDS
TIN FLUORIDES
TIN HALIDES
TIN PHOSPHATES
UPTAKE
YEARS LIVING RADIOISOTOPES