Tc-99m labeled leukocytes: preparation and use in identification of abscess and tissue rejection

Journal Article · Thu Sep 01 04:00:00 EDT 1983 · Radiology; (United States)

DOI:https://doi.org/10.1148/radiology.148.3.6308712· OSTI ID:5546721

Farid, N A; White, S M; Heck, L L; Van Hove, E D

A simple and reproducible method for the preparation and labeling of leukocytes with Tc-99m has been developed. Leukocytes were separated from blood, incubated with stannous pyrophosphate, and then with 20-30 mCi (740-1110 MBq) of /sup 99m/TcO-4. In leukocytes separated from human blood, the labeling efficiency was 81% +/- 6% (n . 4). Experiments on dogs with abscesses showed accumulation of the Tc-99m-labeled leukocytes in the infected sites, indicating the viability of the labeled leukocytes. Additional studies showed that rat lymphocytes that were labeled with Tc-99m, using the same technique, localized in heart transplant tissue that was being rejected.

• Research Organization: Department of Nuclear Medicine, Methodist Hospital, Indiana, Indianapolis
• OSTI ID: 5546721
• Journal Information: Radiology; (United States), Journal Name: Radiology; (United States) Vol. 148:3; ISSN RADLA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ABSCESSES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
DIAGNOSTIC USES
DOGS
GRAFT-HOST REACTION
HOURS LIVING RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
ISOMERIC NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LABELLING
LEUKOCYTES
MAMMALS
MAN
MATERIALS
NUCLEI
ODD-EVEN NUCLEI
OXYGEN COMPOUNDS
PATHOLOGICAL CHANGES
PERTECHNETATES
PHOSPHATES
PHOSPHORUS COMPOUNDS
PRIMATES
RADIOISOTOPES
RATS
RODENTS
TECHNETIUM 99
TECHNETIUM COMPOUNDS
TECHNETIUM ISOTOPES
TIN COMPOUNDS
TIN PHOSPHATES
TRANSITION ELEMENT COMPOUNDS
USES
VERTEBRATES
YEARS LIVING RADIOISOTOPES