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Tc-99m labeled leukocytes: preparation and use in identification of abscess and tissue rejection

Journal Article · · Radiology; (United States)
; ; ;
A simple and reproducible method for the preparation and labeling of leukocytes with Tc-99m, has been developed. Leukocytes were separated from blood, incubated with stannous pyrophosphate, and then with 20-30 mCi (740-1110 M Bq) of /sup 99m/TcO/sub 4//sup -/. In leukocytes separated from human blood, the labeling efficiency was 81% +/- 6% (n=4). Experiments on dogs with abscesses showed accumulation of the Tc-99m-labeled leukocytes in the infected sites, indicating the viability of the labeled leukocytes. Additional studies showed that rat lymphocytes that were labeled with Tc-99m, using the same technique, localized in heart transplant tissue that was being rejected.
Research Organization:
Methodist Hospital of Indiana, Indianapolis
OSTI ID:
6582770
Journal Information:
Radiology; (United States), Journal Name: Radiology; (United States) Vol. 148:3; ISSN RADLA
Country of Publication:
United States
Language:
English

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Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
550900 -- Pathology
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ABDOMEN
ABSCESSES
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL ACCUMULATION
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY AREAS
BODY FLUIDS
CARDIOVASCULAR SYSTEM
CHALCOGENIDES
CHEMICAL PREPARATION
CONNECTIVE TISSUE CELLS
COUNTING TECHNIQUES
DIAGNOSIS
DOGS
GRAFT-HOST REACTION
HEART
HOURS LIVING RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
ISOMERIC NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MAN
MATERIALS
NUCLEI
ODD-EVEN NUCLEI
ORGANS
OXIDES
OXYGEN COMPOUNDS
PATHOLOGICAL CHANGES
PHOSPHORUS COMPOUNDS
PRIMATES
PYROPHOSPHATES
RADIOISOTOPE SCANNING
RADIOISOTOPES
RATS
RODENTS
SOMATIC CELLS
SYNTHESIS
TECHNETIUM 99
TECHNETIUM COMPOUNDS
TECHNETIUM ISOTOPES
TECHNETIUM OXIDES
TRANSITION ELEMENT COMPOUNDS
TRANSPLANTS
VERTEBRATES
YEARS LIVING RADIOISOTOPES