Laser-based multiparameter system for automatic sorting of microscopic particles

Journal Article · Tue Feb 01 04:00:00 EST 1983 · IEEE Trans. Nucl. Sci.; (United States)

DOI:https://doi.org/10.1109/TNS.1983.4332342· OSTI ID:5942768

Turko, B; Leskovar, B

The design, characteristics and experimental results of a Laser-Based Multiparameter system for automatically sorting microscopic particles are described. The system is used mostly for the analysis of biological cells which have been stained with fluorescent dyes and suspended in liquids. The cells, passing in single file through an elliptically focused laser beam, emit fluorescent signals, which are detected by sensors and processed, first in analog fashion and then digitally by a multichannel pulse-height analyzer. The two-color fluorescent and light scatter signals give certain parameters of the particles being measured. Sorting rates of several hundred per second are attainable. The high resolution and good stability of the system ensure fast, reliable and statistically meaningful measurement of particle populations.

• Research Organization: University of California, Berkeley, California 94720
• DOE Contract Number: AC03-76SF00098
• OSTI ID: 5942768
• Journal Information: IEEE Trans. Nucl. Sci.; (United States), Journal Name: IEEE Trans. Nucl. Sci.; (United States) Vol. 30:1; ISSN IETNA
• Country of Publication: United States
• Language: English

Related Subjects

42 ENGINEERING
420300* -- Engineering-- Lasers-- (-1989)
550300 -- Cytology
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
AUTOMATION
DESIGN
ELECTRONIC EQUIPMENT
EQUIPMENT
FLUORESCENCE
LASERS
LUMINESCENCE
MEDICINE
MICROSCOPY
MULTI-PARAMETER ANALYSIS
NUCLEAR MEDICINE
PARTICLE SIZE CLASSIFIERS
PULSE ANALYZERS
PULSES
RESOLUTION
SORTING
STABILITY