Laser-based multiparameter system for automatic sorting of microscopic particles
The design, characteristics and experimental results of a Laser-Based Multiparameter system for automatically sorting microscopic particles are described. The system is used mostly for the analysis of biological cells which have been stained with fluorescent dyes and suspended in liquids. The cells, passing in single file through an elliptically focused laser beam, emit fluorescent signals, which are detected by sensors and processed, first in analog fashion and then digitally by a multichannel pulse-height analyzer. The two-color fluorescent and light scatter signals give certain parameters of the particles being measured. Sorting rates of several hundred per second are attainable. The high resolution and good stability of the system ensure fast, reliable and statistically meaningful measurement of particle populations.
- Research Organization:
- Lawrence Berkeley Lab., CA (USA)
- DOE Contract Number:
- AC03-76SF00098
- OSTI ID:
- 6577265
- Report Number(s):
- LBL-7520-Rev.; CONF-821011-30-Rev.; ON: DE83004189
- Resource Relation:
- Conference: IEEE nuclear science symposium, Washington, DC, USA, 20 Oct 1982; Other Information: Portions of document are illegible
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
AEROSOL MONITORING
AUTOMATION
PARTICLE SIZE
BIOASSAY
AQUEOUS SOLUTIONS
BIOLOGICAL MATERIALS
LASERS
PARTICULATES
DISPERSIONS
MATERIALS
MIXTURES
MONITORING
PARTICLES
SIZE
SOLUTIONS
560301* - Chemicals Metabolism & Toxicology- Cells- (-1987)