Multiparameter cell sorting and analysis
From 1st symposium on mammalian cells; probes and problems; Los Alamos, New Mexico, USA (17 Oct 1973). Improved multisensor cell sorting instrumentation for quantitative analysis and sorting of cells has been developed. Cells stained with fluorescent dyes enter a flow chamber where cell volume, fluorescence, and light scatter sensors simultaneously measure multiple cellular propenties. Cells then emerge in a liquid jet that is broken into uniform liquid droplets. Sensor signals are electronically precessed in one of several ways and are displayed as pulse-amplitude frequency distribution histograms using a multichannel pulse- height analyzer. Processed signals activate cell sorting according to preselected parametric criteria by electrically charging droplets containing cells and electrostatically deflecting them into collection vessels. Illustrative examples of multiparameter cell analysis and sorting experiments using cultured mammalian cells, a model mouse tumor cell system, and animal leukocytes are presented. (auth)
- Research Organization:
- Los Alamos Scientific Lab., N.Mex. (USA)
- DOE Contract Number:
- W-7405-ENG-36
- NSA Number:
- NSA-29-004758
- OSTI ID:
- 4393815
- Report Number(s):
- LA-UR--73-1594; CONF-731007--1
- Country of Publication:
- United States
- Language:
- English
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