Improved multilaser/multiparameter flow cytometer for analysis and sorting of cells and particles

Journal Article · Fri Nov 01 04:00:00 EST 1991 · Review of Scientific Instruments; (United States)

DOI:https://doi.org/10.1063/1.1142210· OSTI ID:5263134

Steinkamp, J A; Habbersett, R C; Hiebert, R D ^[1]

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1. Los Alamos National Laboratory, P.O. Box 1663, Los Alamos, New Mexico (USA)

An improved multilaser instrument has been developed for quantitative analysis and separation of biological cells and particles. Argon ion, krypton ion, and dye lasers are employed as excitation sources to sequentially illuminate cells labeled with multiple fluorochromes as they pass through an improved flow chamber that incorporates an electronic cell-volume sensor and an optical measurement region. Detectors located on the axis of each excitation beam are used to measure axial light loss and forward light scatter. Multicolor fluorescence is measured using a five-channel detector located orthogonal to the laser beam(s)-cell stream intersection(s). Sequential measurements are made on a cell-by-cell basis to provide pulse height, area, and width signals that are made coincident in time by analog delay modules to increase data throughput. Analog electronics are used to compute real-time ratios, sums, and differences of signals. Up to eight signals are acquired and displayed as single-parameter frequency distribution histograms and bivariate diagrams using a microcomputer. Processed signals also activate cell sorting according to computer-controlled preselected parametric criteria. The unique measurement capabilities and other new features designed into this instrument represent marked technological improvements over our previous system.

• OSTI ID: 5263134
• Journal Information: Review of Scientific Instruments; (United States), Journal Name: Review of Scientific Instruments; (United States) Vol. 62:11; ISSN RSINA; ISSN 0034-6748
• Country of Publication: United States
• Language: English

Related Subjects

560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
CELL CULTURES
CELL FLOW SYSTEMS
CYTOLOGICAL TECHNIQUES
DESIGN
ELECTROMAGNETIC RADIATION
LASER RADIATION
PERFORMANCE
RADIATIONS
SORTING