Dual beam sorting at Livermore. [Dual-beam flow cytometer]
Conference
·
OSTI ID:5827797
Most flow cytometers in use today utilize single excitation sources to excite single fluorescent dyes that probe some cellular property or component of interest. To provide for the flexible use of two dyes for the simultaneous measurement of two or more components we have constructed a dual beam flow cytometer/sorter. Argon and krypton ion lasers are used to provide two elliptically shaped beams; cells flow through the beams sequentially. The light collection optics permits the measurement of four variables for each cell. Data acquisition and analysis are acomplished through the use of a computer-based pulse height analyzer.
- Research Organization:
- California Univ., Livermore (USA). Lawrence Livermore Lab.
- DOE Contract Number:
- W-7405-ENG-48
- OSTI ID:
- 5827797
- Report Number(s):
- UCRL-83374; CONF-790643-3
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
440300 -- Miscellaneous Instruments-- (-1989)
47 OTHER INSTRUMENTATION
550300* -- Cytology
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ARGON IONS
CELL FLOW SYSTEMS
CHARGED PARTICLES
CHROMOSOMES
COMPUTERS
DATA ACQUISITION
DATA ANALYSIS
DNA
DYES
ELECTRONIC CIRCUITS
ELECTRONIC EQUIPMENT
FLUORESCENCE
IONS
KRYPTON IONS
LASERS
LUMINESCENCE
MEASURING METHODS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PULSE ANALYZERS
SORTING
47 OTHER INSTRUMENTATION
550300* -- Cytology
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ARGON IONS
CELL FLOW SYSTEMS
CHARGED PARTICLES
CHROMOSOMES
COMPUTERS
DATA ACQUISITION
DATA ANALYSIS
DNA
DYES
ELECTRONIC CIRCUITS
ELECTRONIC EQUIPMENT
FLUORESCENCE
IONS
KRYPTON IONS
LASERS
LUMINESCENCE
MEASURING METHODS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PULSE ANALYZERS
SORTING