Radioimmunoassay T-2 toxin in biological fluids
Conference
·
· J. Assoc. Off. Anal. Chem.; (United States)
OSTI ID:5926136
A simple radioimmunoassay (RIA) for detection of T-2 toxin in biological fluids was developed. The method, which involves a Sep-Pak C/sub 18/ reverse phase column cleanup step before RIA, can detect 0.5 ppb T-2 toxin in serum and 2.5 ppb T-2 toxin in urine and milk. Recoveries for toxin added to serum and urine or milk samples were 87-106% in the 0.5-20 ppb range and 88-117% in the 2.5-20 ppb range, respectively. If samples were not cleaned up, false positive results prevented positive detection at concentrations less than 2.5 ppb in urine and milk.
- Research Organization:
- Univ. of Wisconsin, Madison
- OSTI ID:
- 5926136
- Conference Information:
- Journal Name: J. Assoc. Off. Anal. Chem.; (United States) Journal Volume: 64:3
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANTIGENS
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BLOOD SERUM
BODY FLUIDS
FOOD
ISOTOPE APPLICATIONS
MATERIALS
MEASURING METHODS
MILK
RADIOASSAY
RADIOIMMUNOASSAY
TOXIC MATERIALS
TOXINS
TRACER TECHNIQUES
URINE
WASTES
62 RADIOLOGY AND NUCLEAR MEDICINE
ANTIGENS
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BLOOD SERUM
BODY FLUIDS
FOOD
ISOTOPE APPLICATIONS
MATERIALS
MEASURING METHODS
MILK
RADIOASSAY
RADIOIMMUNOASSAY
TOXIC MATERIALS
TOXINS
TRACER TECHNIQUES
URINE
WASTES