Homologous radioimmunoassay for guinea pig corticosteroid-binding globulin
A rapid, specific, and sensitive (requiring only 20 fmole of antigen equivalent to 0.007) l of serum) radioimmunoassay (RIA) was developed for the measurement of guinea pig corticosteroid-binding globulin (CBG). CBG was purified to homogeneity from guinea pig serum by affinity chromatography and used for immunization, as the standard and as the radiolabeled trace in the RIA. The antiserum to CBG was raised in rabbits. It was judged specific by immunoelectrophoresis and by comparison of RIA values with steroid-binding assay profiles obtained on serum separated on the basis of size and ion-exchange properties. The results of the radioimmunoassays agree with those of a steroid-binding assay run on identical samples. The sensitivity of the assay allows detection of CBG in serial serum samples, other biologic fluids such as milk, and cell culture supernatants.
- Research Organization:
- Univ. of California, San Francisco
- OSTI ID:
- 6157338
- Journal Information:
- Proc. Soc. Exp. Biol. Med.; (United States), Journal Name: Proc. Soc. Exp. Biol. Med.; (United States) Vol. 185:3; ISSN PSEBA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Biological half-life and transfer of maternal corticosteroid-binding globulin to amniotic fluid in the rabbit
A radioimmunoassay for lignin in plant cell walls
Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ADRENAL HORMONES
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MODELS
BLOOD SERUM
CORTICOSTEROIDS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
GLOBULINS
GUINEA PIGS
HORMONES
HYDROXY COMPOUNDS
IMMUNOASSAY
IMMUNOLOGY
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KETONES
LABELLED COMPOUNDS
MAMMALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PREGNANCY
PREGNANES
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RADIOISOTOPES
RODENTS
SPECIFICITY
STEROID HORMONES
STEROIDS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
USES
VERTEBRATES