Studies on the immunochemical techniques for detection of selected fungal and dinoflagellate toxins. Annual progress report No. 2, 1 August 1982-31 July 1983

Technical Report · Mon Aug 15 04:00:00 EDT 1983

OSTI ID:5739504

Chu, F S

During the second year (August 1, 1982 to July 31, 1983), conditions for improving antibody production against T-2 toxin were studied. T-2 HS-BSA which has 10-15 moles of T-2 per mole of BSA was found to be a better immunogen than T-2-HG-BSA. Antibody titers as high as 11,000-14,000 were obtained from rabbits after repeated booster injections. High specific radioactive T-2, DAS, DOVE were prepared. With combination of the high specific radioactive T-2 toxin and high titer antibody, as little as 25 pg of T-2 toxin can be detected by the RIA in each assay. An indirect ELISA which permits detection of 0.2-1 ppb of T-2 toxin in urine, serum and milk was also established. Antibody against T-2 toxin was used to localize T-2 toxin in different tissues and organs of mice by an immunohistochemical technique. A collaborative study with Dr. Hunter revealed that antibody titers of T-2 toxin increased drastically in an immunomodulation system in mice. Titers as high as 50,000 were obtained in mice 10 days after immunization with T-2-HS-IgG (goat). Antibodies against diacetoxyscirpenol (DAS), and deoxyverrucarol (DOVE) were obtained from rabbits after immunizing with DAS-hemisuccinate (HS)-BSA, DAS-hemiglutarate (HG)-BSA and DOVE-HS-BSA. DAS-HG-BSA was found to be a better immunogen than DAS-HS-BSA for the production of antibody against DAS. The antibody for DAS is most specific for DAS. However, DOVE antidbodies were found to be less specific. Radioimmunoassays for both DAS and DOVE were established, and the detection limits were found.

• Research Organization: Wisconsin Univ., Madison (USA)
• OSTI ID: 5739504
• Report Number(s): AD-A-165845/9/XAB
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
550900* -- Pathology
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ANTIBODIES
ANTIGENS
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BODY FLUIDS
DETECTION
FOOD
FUNGI
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
MAMMALS
MATERIALS
MICE
MILK
PLANTS
PRODUCTION
RABBITS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RODENTS
TOXIC MATERIALS
TOXINS
TRACER TECHNIQUES
URINE
VERTEBRATES
WASTES