Immunological techniques for detection of fungal and dinoflagellate toxins. Annual report, 1 May 1987-30 April 1988

Technical Report · Thu May 12 04:00:00 EDT 1988

OSTI ID:6746425

Chu, F S

1) A new direct ELISA, which involves the use of a generic type antibody for T-2 toxin and 3-acetyl-neosolaniol-hemisuccinate (3-Ac-NEOS-HS) conjugated to horseradish peroxidase for the analysis of T-2 toxin metabolites, was established. 2) Analysis of 5 metabolites, i.e., T-2 toxin, HT-2, T-2-4ol, 3'-OH-HT-2, NEOS and a mixture of these five toxins, added at toxin concentrations of 50 and 200 ng/mL of urine, revealed that 87% of the added toxins were recovered analytically at both concentrations in the ELISA, and 69% in RIA and the 200 ppb level. 3) A series of monkey urine samples obtained from a metabolic study was subjected to the new ELISA protocol was as well as to RIA. 4) An extensive study to evaluate a fluorometric method, i.e., hit-and-run assay was made. 5) A direct ELISA for deoxynivalenol (DON) was established. 6) Further improvements for the ELISA for saxitoxin were made. 7) New approaches to produce antibody against nivalenol and saxitoxin were made, but the antibody titers were low. 8) Antibody against microcystin (MCS) was obtained from rabbits after immunizing the animals with MCS-BSA conjugate. 9) A chemical method for the preparation of different monoacetoxyscirpenol (MAS) was established. 10) Routine preparation of different immunochemical reagents continued. Phytotoxins Biological warfare agents; Dinoflagellates. (AW)

• Research Organization: Bundesministerium fuer Wirtschaftliche Zusammenarbeit, Bonn (Germany, F.R.)
• OSTI ID: 6746425
• Report Number(s): AD-A-195782/8/XAB
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
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59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ANTIBODIES
ANTIGENS
BIOLOGICAL MATERIALS
BIOLOGICAL WARFARE AGENTS
BIOLOGICAL WASTES
BODY FLUIDS
CHEMICAL ANALYSIS
DETECTION
DOCUMENT TYPES
ENZYMES
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
MAMMALS
MATERIALS
METABOLISM
MONKEYS
OXIDOREDUCTASES
PEROXIDASES
PRIMATES
PROGRESS REPORT
RABBITS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
TITRATION
TOXIC MATERIALS
TOXINS
TRACER TECHNIQUES
URINE
VERTEBRATES
WASTES
WEAPONS