Effect of benzo(a)pyrene-specific antibodies on the disposition of benzo(a)pyrene

Thesis/Dissertation · Sat Jan 01 04:00:00 EST 1983

OSTI ID:5232000

Kantrowitz, J D

The disposition of benzo(a)pyrene (BP) in mice can be altered by the administration of anti-BP antibodies. Antibodies specific for BP were produced in rabbits by administration of a BP-bovine serum albumin (BSA) conjugate. Antibodies with a high specificity for BP were obtained using this conjugate after 2-3 months of immunization. Competitive radioimmunoassay experiments showed unlabeled BP to have a greater ability to displace /sup 3/H-BP from antibody-binding sites than a series of BP metabolites and structural analogs. Following the intravenous administration of /sup 3/H-BP concomitantly with rabbit antiserum or normal rabbit serum (NRS), there was a more than 7-fold increase in the plasma level of /sup 3/H-BP in immunized mice than controls. While the decline in radioactivity in both groups of mice had two phases, the beta phase of elimination was much shorter in immunized mice (t/sub 1/2/ of 11.4 h) than in controls (t/sub 1/2/ of 20.1 h). A two-compartment model, constructed for BP, indicated a reduction in the total volume of distribution (V/sub d/) of BP as well as a decrease in its V/sub d/ in the central and peripheral compartments. In studies with mice passively immunized with the rabbit antiserum, their in vivo binding capacity decayed with a t/sub 1/2/ of 4.9 days. Assay of tissues from mice which had received /sup 3/H-BP intravenously showed that the tissue:plasma ratio for BP was less in immunized mice than controls.

• Research Organization: University of Medicine and Dentistry of New Jersey, Piscataway (USA). Rutgers Medical School
• OSTI ID: 5232000
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
560305 -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
62 RADIOLOGY AND NUCLEAR MEDICINE
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
ANTIBODIES
AROMATICS
BENZOPYRENE
BIOLOGICAL EFFECTS
CHROMATOGRAPHY
CONDENSED AROMATICS
DISTRIBUTION
HYDROCARBONS
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIQUID COLUMN CHROMATOGRAPHY
MAMMALS
MICE
ORGANIC COMPOUNDS
PHARMACOLOGY
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RODENTS
SEPARATION PROCESSES
TISSUE DISTRIBUTION
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES