Quantitation of exposure to benzo(a)pyrene with monoclonal antibodies

Journal Article · Tue Oct 01 04:00:00 EDT 1985 · Environ. Health Perspect.; (United States)

DOI:https://doi.org/10.1289/ehp.856295· OSTI ID:5446683

Santella, R M; Hsieh, L L; Lin, C D; Viet, S; Weinstein, I B

It is now possible to quantitate carcinogen adducts on DNA by highly sensitive immunoassays. These techniques are particularly useful for screening human populations for exposure to potential environmental carcinogens. The authors have developed a panel of monoclonal antibodies that react with benzo(a)pyrene (BP) modified DNA to be used in an enzyme linked immunoassay (ELISA) to quantitate adduct levels of both human and animal samples. BALBc/Cr mice were immunized with either DNA modified by 7..beta..,8..cap alpha..-dihydroxy-9..cap alpha..,10..cap alpha..-epoxy-7,8,9,10,-tetrahydrobenzo(a)pyrene (BPDE-I-DNA) complexed electrostatically to methylated bovine serum albumin or with BPDE-I-modified guanosine conjugated with bovine serum albumin (BPDE-I-G-BSA). Four stable clones were produced from the spleen cells of animals immunized with BPDE-I-DNA and one from BPDE-I-G-BSA immunized animals. All antibodies were shown to be highly specific for BPDE-I-DNA and did not crossreact with nonmodified DNA or with N-2-acetylaminofluorene or 1-aminopyrene modified DNA. In general, all the antibodies showed the greatest affinity for their original antigen. These antibodies are currently being used in a highly sensitive competitive ELISA to quantitate levels of BP-DNA adducts in various animal and human tissue samples.

• Research Organization: Columbia Univ., New York, NY
• OSTI ID: 5446683
• Journal Information: Environ. Health Perspect.; (United States), Journal Name: Environ. Health Perspect.; (United States) Vol. 62; ISSN EVHPA
• Country of Publication: United States
• Language: English

Related Subjects

560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADDUCTS
ANIMALS
ANTIBODIES
AROMATICS
BENZOPYRENE
BIOLOGICAL EFFECTS
CONDENSED AROMATICS
DNA ADDUCTS
ENZYME IMMUNOASSAY
HYDROCARBONS
IMMUNOASSAY
IMMUNOLOGY
MAMMALS
MICE
MONOCLONAL ANTIBODIES
ORGANIC COMPOUNDS
RODENTS
VERTEBRATES