Effect of ethylene action inhibitors upon wound-induced gene expression in tomato pericarp
Journal Article
·
· Plant Physiology; (USA)
- Purdue Univ., West Lafayette, IN (USA)
The contribution of wound-ethylene to wound-induced gene expression was investigated in unripe tomato pericarp using inhibitors of ethylene action. Wounded unripe tomato pericarp was treated with 2,5-norbornadiene or silver thiosulfate to inhibit specifically the induction of ethylene-dependent mRNA species. Poly(A){sup +} RNAs isolated from these tissues after 12 hours of wounding were translated in vitro in a rabbit reticulocyte lysate system and ({sup 35}S)methionine-labeled polypeptides were compared to unwounded controls after separation by one and two-dimensional polyacrylamide gel electrophoresis. Results show that mechanical wounding induces a dramatic shift in gene expression (over 50 mRNA species) but expression of less than 15% of these genes is affected by the treatment with ethylene action inhibitors. A selective decrease in mRNAs coding for a 37 kilodalton doublet and 75 kilodalton polypeptides is observed in 2,5-norbornadiene and silver thiosulfate treated wounded pericarp. Levels of hydroxyproline-rich glycoprotein mRNAs induced in wounded tissue were not influenced by inhibitors of ethylene action.
- OSTI ID:
- 5922794
- Journal Information:
- Plant Physiology; (USA), Journal Name: Plant Physiology; (USA) Vol. 91:1; ISSN 0032-0889; ISSN PLPHA
- Country of Publication:
- United States
- Language:
- English
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