Expression of alternative oxidase in tomato

Conference · Tue May 01 04:00:00 EDT 1990 · Plant Physiology, Supplement; (USA)

OSTI ID:5725034

Kakefuda, M; McIntosh, L ^[1]

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1. Michigan State Univ., East Lansing (USA)

Tomato fruit ripening is characterized by an increase in ethylene biosynthesis, a burst in respiration (i.e. the climacteric), fruit softening and pigmentation. As whole tomatoes ripened from mature green to red, there was an increase in the alternative oxidase capacity. Aging pink tomato slices for 24 and 48 hrs also showed an increase of alternative oxidase and cytochrome oxidase capacities. Monoclonal antibodies prepared to the Sauromatum guttatum alternative oxidase were used to follow the appearance of alternative oxidase in tomato fruits. There is a corresponding increase in a 36kDa protein with an increase in alternative oxidase capacity. Effects of ethylene and norbornadiene on alternative oxidase capacity were also studied. We are using an alternative oxidase cDNA clone from potato to study the expression of mRNA in ripening and wounded tomatoes to determine if the gene is transcriptionally regulated.

• OSTI ID: 5725034
• Report Number(s): CONF-9007196--
• Conference Information: Journal Name: Plant Physiology, Supplement; (USA) Journal Volume: 93:1
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ALKENES
ANTIBODIES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
CYTOCHROME OXIDASE
ENZYMES
ETHYLENE
FOOD
FRUITS
GROWTH
HAEM DEHYDROGENASES
HERBS
HYDROCARBONS
KINETICS
MESSENGER-RNA
MONOCLONAL ANTIBODIES
NUCLEIC ACIDS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PLANT GROWTH
PLANTS
REACTION KINETICS
RNA
TOMATOES