Cloning and expression of the potato alternative oxidase gene
Conference
·
· Plant Physiology, Supplement; (USA)
OSTI ID:5851044
- MSU-DOE Plant Research Laboratory, East Lansing, MI (USA) Michigan State Univ., East Lansing (USA)
Mitochondria from 24-hour-aged potato slices possess an alternative path capacity and a 36kD protein not present in fresh potato mitochondria. This 36kD protein was identified by a monoclonal antibody against the Sauromatum guttatum alternative oxidase. These results suggest de novo synthesis of the 36kD protein during the aging process. To investigate this phenomenon, a clone containing a potato alternative oxidase gene was isolated from a cDNA library using the S. guttatum gene as a probe. This clone shows areas of high homology to the S. guttatum gene. Norther blots of RNA from fresh and 24-hour-aged potato slices are being probed with the potato gene to examine its expression in relation to the appearance of the 36kD protein.
- OSTI ID:
- 5851044
- Report Number(s):
- CONF-9007196--
- Conference Information:
- Journal Name: Plant Physiology, Supplement; (USA) Journal Volume: 93:1
- Country of Publication:
- United States
- Language:
- English
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