Wound-induced changes in mRNA populations in tomato pericarp tissue

Conference · Wed Apr 01 04:00:00 EST 1987 · Plant Physiol., Suppl.; (United States)

OSTI ID:5878315

Henstrand, J M; Handa, A K

Immature green tomato pericarp tissue was wounded by cutting into small pieces. At various intervals, ethylene production was monitored and the corresponding tissue harvested for mRNA extraction. Poly (A)/sup +/ RNA was fractionated from total RNA using oligo (dT)-cellulose chromatography and was translated in vitro in a rabbit reticulocyte lysate system using /sup 35/S-methionine. Labeled products were subjected to one and two dimensional polyacrylamide gel electrophoresis (PAGE) to analyze wound-induced changes in mRNA populations. Analyses of autoradiograms of corresponding single dimension SDS-PAGE showed changes in at least 12 major polypeptides with 6 declining (18, 19, 24, 36, 44, 69 kD) and 6 increasing (21, 41, 46, 54, 75, > 94 kD) after wounding. Among the polypeptides resolved (over 200) on two dimensional PAGE, at least 15 showed dramatic increases in the wounded tissue. Results indicate that wounding of tomato pericarp causes induction of synthesis and accumulation of several mRNA species while inhibiting production of relatively few mRNA species.

• Research Organization: Purdue Univ., West Lafayette, IN
• OSTI ID: 5878315
• Report Number(s): CONF-8707108-
• Conference Information: Journal Name: Plant Physiol., Suppl.; (United States) Journal Volume: 83:4
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKENES
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOSYNTHESIS
BODY
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
ELECTROPHORESIS
ETHYLENE
EVEN-ODD NUCLEI
FOOD
FRUITS
GENE REGULATION
HYDROCARBONS
INJURIES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
MESSENGER-RNA
NUCLEI
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PEPTIDES
PLANT TISSUES
POLYPEPTIDES
PROTEINS
RADIOISOTOPES
RNA
SEPARATION PROCESSES
SULFUR 35
SULFUR ISOTOPES
SYNTHESIS
TISSUES
TOMATOES
TRACER TECHNIQUES
WOUNDS