Peroxidase gene expression during tomato fruit ripening
Auxin oxidation has been reported to play a critical role in the initiation of pear fruit ripening and a tomato fruit peroxidase (POD) has been shown to have IAA-oxidase activity. However, little is known about changes in the expression of POD mRNA in tomato fruit development. They are investigating the expression of POD mRNA during tomato fruit maturation. Fruit pericarp tissues from six stages of fruit development and ripening (immature green, mature green, breaker, turning, ripe, and red ripe fruits) were used to extract poly (A)/sup +/ RNAs. These RNAs were translated in vitro in a rabbit reticulocyte lysate system using L-/sup 35/S-methionine. The /sup 35/S-labeled products were immunoprecipitated with POD antibodies to determine the relative proportions of POD mRNA. High levels of POD mRNA were present in immature green and mature green pericarp, but declined greatly by the turning stage of fruit ripening. In addition, the distribution of POD mRNA on free vs bound polyribosomes will be presented, as well as the presence or absence of POD mRNA in other tomato tissues.
- Research Organization:
- Purdue Univ., West Lafayette, IN
- OSTI ID:
- 5964468
- Report Number(s):
- CONF-8707108-
- Journal Information:
- Plant Physiol., Suppl.; (United States), Journal Name: Plant Physiol., Suppl.; (United States) Vol. 83:4; ISSN PPYSA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
AUXINS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL FUNCTIONS
BODY
CELL CONSTITUENTS
DAYS LIVING RADIOISOTOPES
ENZYMES
EVEN-ODD NUCLEI
FOOD
FRUITS
FUNCTIONS
GENE REGULATION
GENES
IMMUNOASSAY
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
MESSENGER-RNA
NUCLEI
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANOIDS
OXIDOREDUCTASES
PEROXIDASES
PHYSIOLOGY
PLANT GROWTH REGULATORS
PLANT TISSUES
RADIOISOTOPES
RIBOSOMES
RIPENING
RNA
SULFUR 35
SULFUR ISOTOPES
TISSUES
TOMATOES
TRACER TECHNIQUES
TRANSCRIPTION