Prolactin-stimulated ornithine decarboxylase induction in rat hepatocytes: Coupling to diacylglycerol generation and protein kinase C
Journal Article
·
· Life Sciences; (USA)
- Kirksville College of Osteopathic Medicine, MO (USA)
The trophic effects of prolactin (PRL) in rat liver have been linked to activation of protein kinase C (PKC). Since alterations in PKC activity imply its activation by 1,2-diacylglycerol (DAG), we tested whether PRL treatment stimulated DAG generation coupled to induction of a growth response in primary hepatocytes. Addition of PRL to hepatocyte cultures significantly increased ({sup 3}H)-glycerol incorporation into DAG within 5 minutes which was followed by a loss of cytosolic PKC activity by 10 minutes. Prolactin also significantly enhanced radiolabel incorporation into triacylglycerol and phospholipids within 10 minutes and induced ODC activity at 6 hours. Therefore, prolactin-stimulated alterations in PKC activity are preceded by enhanced DAG generation. Moreover, these events appear to be coupled to PRL-stimulated entry of hepatocytes into cell cycle.
- OSTI ID:
- 5922656
- Journal Information:
- Life Sciences; (USA), Journal Name: Life Sciences; (USA) Vol. 48:3; ISSN LIFSA; ISSN 0024-3205
- Country of Publication:
- United States
- Language:
- English
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Thu May 01 00:00:00 EDT 1986
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Thesis/Dissertation
·
Sat Dec 31 23:00:00 EST 1988
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OSTI ID:6528660
Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALCOHOLS
AMINO ACIDS
ANIMAL CELLS
ANIMALS
BIOCHEMICAL REACTION KINETICS
BIOSYNTHESIS
CARBON-CARBON LYASES
CARBOXY-LYASES
CARBOXYLIC ACIDS
CELL CYCLE
DECARBOXYLASES
ENZYME ACTIVITY
ENZYME INDUCTION
ENZYMES
GENE REGULATION
GLYCEROL
GONADOTROPINS
GROWTH
HORMONES
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
LIVER CELLS
LTH
LYASES
MAMMALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORNITHINE
PEPTIDE HORMONES
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
PITUITARY HORMONES
RATS
REACTION KINETICS
RODENTS
SOMATIC CELLS
SYNTHESIS
TRACER TECHNIQUES
TRANSFERASES
TRITIUM COMPOUNDS
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
ALCOHOLS
AMINO ACIDS
ANIMAL CELLS
ANIMALS
BIOCHEMICAL REACTION KINETICS
BIOSYNTHESIS
CARBON-CARBON LYASES
CARBOXY-LYASES
CARBOXYLIC ACIDS
CELL CYCLE
DECARBOXYLASES
ENZYME ACTIVITY
ENZYME INDUCTION
ENZYMES
GENE REGULATION
GLYCEROL
GONADOTROPINS
GROWTH
HORMONES
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
LIVER CELLS
LTH
LYASES
MAMMALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORNITHINE
PEPTIDE HORMONES
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
PITUITARY HORMONES
RATS
REACTION KINETICS
RODENTS
SOMATIC CELLS
SYNTHESIS
TRACER TECHNIQUES
TRANSFERASES
TRITIUM COMPOUNDS
VERTEBRATES