Endothelin stimulates a sustained 1,2-diacylglycerol increase and protein kinase C activation in bovine aortic smooth muscle cells
Journal Article
·
· Biochem. Biophys. Res. Commun.; (United States)
Endothelin is a long-lasting potent vasoconstrictor peptide. We report here that in bovine aortic smooth muscle cells, endothelin biphasically increased total cellular diacylglycerol (DAG) content. When cellular DAG was labeled with (/sup 14/C) glycerol for 48h, endothelin stimulated (/sup 14/C)DAG formation in a biphasic pattern. Only one prolonged phase of DAG accumulation was observed when cells were labeled with (/sup 3/H)glycerol for 2 h. Endothelin induced an increase in the membranous protein kinase C (PKC) activities, which lasted for more than 20 min. These data suggest that (i) endothelin stimulates a sustained generation of DAG, (ii) this accumulation of DAG results in a sustained translocation of cytosolic PKC activities to the membrane.
- Research Organization:
- Brigham Women's Hospital, Boston, MA (USA)
- OSTI ID:
- 5822836
- Journal Information:
- Biochem. Biophys. Res. Commun.; (United States), Journal Name: Biochem. Biophys. Res. Commun.; (United States) Vol. 162:1; ISSN BBRCA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALCOHOLS
ANIMALS
AORTA
ARTERIES
BIOCHEMICAL REACTION KINETICS
BIOSYNTHESIS
BLOOD VESSELS
BODY
CARBON 14 COMPOUNDS
CARDIOVASCULAR AGENTS
CARDIOVASCULAR SYSTEM
CATTLE
CELL CONSTITUENTS
CELL CULTURES
CELL MEMBRANES
DOMESTIC ANIMALS
DRUGS
ENZYME ACTIVITY
ENZYMES
GLYCEROL
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
MAMMALS
MEMBRANES
MUSCLES
ORGANIC COMPOUNDS
ORGANS
PEPTIDES
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
PROTEINS
REACTION KINETICS
RUMINANTS
SYNTHESIS
TRACER TECHNIQUES
TRANSFERASES
TRITIUM COMPOUNDS
VASOCONSTRICTORS
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
ALCOHOLS
ANIMALS
AORTA
ARTERIES
BIOCHEMICAL REACTION KINETICS
BIOSYNTHESIS
BLOOD VESSELS
BODY
CARBON 14 COMPOUNDS
CARDIOVASCULAR AGENTS
CARDIOVASCULAR SYSTEM
CATTLE
CELL CONSTITUENTS
CELL CULTURES
CELL MEMBRANES
DOMESTIC ANIMALS
DRUGS
ENZYME ACTIVITY
ENZYMES
GLYCEROL
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
MAMMALS
MEMBRANES
MUSCLES
ORGANIC COMPOUNDS
ORGANS
PEPTIDES
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
PROTEINS
REACTION KINETICS
RUMINANTS
SYNTHESIS
TRACER TECHNIQUES
TRANSFERASES
TRITIUM COMPOUNDS
VASOCONSTRICTORS
VERTEBRATES