Monoclonal antibody-directed phenotyping of cytochrome P-450-dependent aryl hydrocarbon hydroxylase and 7-ethoxycoumarin deethylase in mammalian tissues
The distribution of cytochromes P-450 that catalyze aryl hydrocarbon hydroxylase and 7-ethoxycoumarin O-deethylase were studied with monoclonal antibody (MAb) 1-7-1 which completely inhibits these activities of a purified 3-methylcholanthrene-induced rat liver cytochrome P-450. The degree of inhibition by MAb 1-7-1 quantitatively assesses the contribution of different cytochromes P-450 in the liver, lung, and kidney microsomes from untreated, 3-methylcholanthrene- and phenobarbital (PB)-treated rats, mice, guinea pigs, and hamsters. Enzyme sensitivity to MAb 1-7-1 inhibition defines two types of cytochrome P-450 contributing to aryl hydrocarbon hydroxylase and 7-ethoxycoumarin O-deethylase. The MAb 1-7-1 sensitive cytochrome P-450 is a major contributor to aryl hydrocarbonhydroxylase in rat liver, lung, and kidney of 3-methylcholanthrene-treated rats, C57BL/6 mice, guinea pigs, and hamsters. 7-Ethoxycoumarin 0-deethylase is also a function of both the MAb 1-7-1-sensitive and insensitive classes of cytochromeP-450. The ratio of the classes contributing to aryl hydrocarbon hydroxylase and 7-ethoxycoumarin O-deethylase differs in the various tissues and species and after inducer treatment. All of the 7-ethoxycoumarin O-deethylase activity in guinea pigs and hamsters is a function of cytochromes P-450 different than the MAb 1-7-1-sensitive cytochrome P-450 responsible for aryl hydrocarbon hydroxylase activity. Thus, the MAb 1-7-1 antigenically defines the type of cytochromes P-450 contributing to each reaction.
- Research Organization:
- National Institutes of Health, Bethesda, MD
- OSTI ID:
- 5904327
- Journal Information:
- J. Biol. Chem.; (United States), Journal Name: J. Biol. Chem.; (United States) Vol. 259:14; ISSN JBCHA
- Country of Publication:
- United States
- Language:
- English
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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANESTHETICS
ANIMALS
ANTIBODIES
ANTICONVULSANTS
AROMATICS
ARYL 4-MONOOXYGENASE
AZINES
BARBITURATES
BIOCHEMICAL REACTION KINETICS
BODY
CELL CONSTITUENTS
CENTRAL NERVOUS SYSTEM DEPRESSANTS
CYTOCHROMES
DIGESTIVE SYSTEM
DRUGS
ENZYME ACTIVITY
ENZYMES
GLANDS
GUINEA PIGS
HAMSTERS
HETEROCYCLIC COMPOUNDS
HYDROCARBONS
HYDROXYLASES
HYPNOTICS AND SEDATIVES
INHIBITION
ISOENZYMES
KIDNEYS
KINETICS
LIVER
LUNGS
MAMMALS
MICE
MICROSOMES
MONOCLONAL ANTIBODIES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANOIDS
ORGANS
OXIDOREDUCTASES
PHENOBARBITAL
PIGMENTS
POLYCYCLIC AROMATIC HYDROCARBONS
PYRIMIDINES
RATS
REACTION KINETICS
RESPIRATORY SYSTEM
RODENTS
TOXICITY
VERTEBRATES