Induction of hepatic drug-metabolizing enzymes in rats treated with 1-nitropyrene

Journal Article · Sun Jan 31 23:00:00 EST 1988 · Environ. Res.; (United States)

DOI:https://doi.org/10.1016/S0013-9351(88)80011-2· OSTI ID:5142592

Belisario, M A; Borgia, R; Pecce, R; de Lorenzo, F

The inducing effects of 1-nitropyrene (1-NP) on the microsomal cytochrome P-450 system were studied in rats. Intraperitoneal administration of 1-NP led to increases in cytochrome P-450 content and aryl hydrocarbon hydroxylase, ethoxycoumarin, and ethoxyresorufin-O-deethylase activities. These increases were does dependent. Cytochrome b/sub 5/ content aminopyrine and p-nitroanisole demethylase activities were not affected by treatment of rats with 1-NP. Substrate specificity, sensitivity to mixed-function oxidase inhibitors, and electrophoretic pattern of 1-NP-induced cytochrome(s) P-450 were compared to the major forms of cytochrome P-450 induced by phenobarbital and methylcholanthrene. Furthermore microsomes from 1-NP-induced rats showed greater ability to metabolize the chemical as compared with those from control animals; this result indicates that 1-NP induces a form(s) of cytochrome P-450 especially effective in the metabolism of the substance itself.

• Research Organization: Istituto di Biochimica Cellulare e Molecolare, Napoli (Italy)
• OSTI ID: 5142592
• Journal Information: Environ. Res.; (United States), Journal Name: Environ. Res.; (United States) Vol. 45:1; ISSN ENVRA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
AROMATICS
BIOLOGICAL ADAPTATION
CELL CONSTITUENTS
CYTOCHROME OXIDASE
DOSE-RESPONSE RELATIONSHIPS
ENZYME ACTIVITY
ENZYME INHIBITORS
ENZYMES
HAEM DEHYDROGENASES
HYDROCARBONS
HYDROXYLASES
MAMMALS
METABOLIC ACTIVATION
METABOLISM
METABOLITES
MICROSOMES
NITRO COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANOIDS
OXIDOREDUCTASES
POLYCYCLIC AROMATIC HYDROCARBONS
RATS
RODENTS
VERTEBRATES