Work-place mixtures and lung-defense systems. Final report

Technical Report · Sat Jan 01 04:00:00 EST 1983

OSTI ID:5119329

Rabovsky, J

The effects of diesel exhaust, coal dust, and n-alkanes on lung and liver enzymes were studied in rats. Male Fischer-344 rats were exposed by inhalation to 1 milligram per cubic meter (mg/m/sup 3/) diesel exhausts or 1 mg/m/sup 3/ coal dust alone or in combination 7 hours daily, 5 days per week for 2 years. Cytochrome-P-450 associated benzo(a)pyrene-hydroxylase (BaP hydroxylase) and 7-ethoxycoumarin-o-deethylase activities were assayed in lung and liver microsomes after 3, 6, or 24 months. Sprague-Dawley-rats that had been pretreated with 0 or 80 mg per kilogram beta-naphthoflavone (BNF) were exposed to 0 to 2 millimolar (mM) concentrations of six straight-chained alkanes. Enzyme activities in lung and liver microsomes were determined. Diesel exhausts plus coal dust caused a slight, statistically insignificant, reduction in lung BaP hydroxylase activity. No other changes in lung and liver activities were observed. In non BNF pretreated rats, liver enzyme activities were decreased by 2mM alkanes, maximal decreases of 79 and 72%, respectively, occurring with n-octane. Lung enzyme activities were not affected. In BNF pretreated rats, lung o-deethylase activity was decreased by 44% by n-octane. The author concludes that enzyme sensitivity depends on the alkane.

• Research Organization: National Inst. for Occupational Safety and Health, Cincinnati, OH (USA)
• OSTI ID: 5119329
• Report Number(s): PB-85-220697/XAB
• Country of Publication: United States
• Language: English

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Atmospheric-- Chemicals Monitoring & Transport-- (-1989)
54 ENVIRONMENTAL SCIENCES
560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AIR POLLUTION
ALKANES
BIOASSAY
BIOLOGICAL EFFECTS
BODY
CARBONACEOUS MATERIALS
CARCINOGENS
CELL CONSTITUENTS
COAL
DIESEL FUELS
DIGESTIVE SYSTEM
DUSTS
ENERGY SOURCES
ENZYME ACTIVITY
ENZYMES
EXHAUST GASES
FLUIDS
FOSSIL FUELS
FUELS
GASEOUS WASTES
GASES
GLANDS
HAZARDOUS MATERIALS
HYDROCARBONS
INDOOR AIR POLLUTION
INDUSTRIAL MEDICINE
INDUSTRIAL WASTES
INSPECTION
LABORATORY ANIMALS
LIVER
LUNGS
MATERIALS
MEDICINE
METABOLISM
MICROSOMES
MUTAGENS
ORGANIC COMPOUNDS
ORGANOIDS
ORGANS
PARTICLES
PETROLEUM PRODUCTS
POLLUTION
RESPIRATORY SYSTEM
TOXICITY
VENTILATION
WASTES