Monoclonal antibody-directed radioimmunoassay of specific cytochromes P-450
A rapid solid phase radioimmunoassay (RIA) for cytochromes P-450 has been developed utilizing specific monoclonal antibodies to major forms of rat liver cytochrome P-450 that are induced by 3-methylcholanthrene (MC-P-450) and phenobarbital (PB-P-450). Monoclonal antibodies (MAbs) that were endogenously labeled with (/sup 35/S)methionine were used to detect MAb-specific cytochromes P-450 in liver microsomes from untreated rats and rats pretreated with 3-methylcholanthrene (MC) or phenobarbital. The competitive binding assays are rapid and can detect cytochrome P-450 in less than 100 ng of microsomal protein. Tthe RIA was used to examine the distribution of MAb-specific cytochromes P-450 in extrahepatic tissues of MC-treated rats; an approximately 30- to 50-fold greater amount of MC-P-450 in liver relative to lung and kidney was observed, which corresponds well with aryl hydrocarbon hydroxylase activity in these tissues. The inducibility of MAb-specific cytochromes P-450 were observed in MC-treated rats, guinea pigs, and C57BL/6 mice, all highly inducible for aryl hydrocarbon hydroxylase; little increase was observed for the relatively noninducible DBA/2 mouse strain.
- Research Organization:
- National Cancer Inst., Bethesda, MD
- OSTI ID:
- 6914308
- Journal Information:
- J. Biol. Chem.; (United States), Journal Name: J. Biol. Chem.; (United States) Vol. 259:3; ISSN JBCHA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Structural characterization of a monoclonal antibody immunopurified pulmonary cytochrome P-450 from 3-methylcholanthrenetreated rats
Immunochemical analysis of cytochromes P-450 in teleosts with polyclonal antibodies to cytochrome P-450E
Related Subjects
560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANESTHETICS
ANIMALS
ANTIBODIES
ANTICONVULSANTS
AROMATICS
AZINES
BARBITURATES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
CENTRAL NERVOUS SYSTEM DEPRESSANTS
CYTOCHROMES
DAYS LIVING RADIOISOTOPES
DRUGS
EVEN-ODD NUCLEI
GUINEA PIGS
HETEROCYCLIC COMPOUNDS
HYDROCARBONS
HYPNOTICS AND SEDATIVES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAMMALS
METABOLIC ACTIVATION
MICE
MONOCLONAL ANTIBODIES
NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PHENOBARBITAL
PIGMENTS
POLYCYCLIC AROMATIC HYDROCARBONS
PYRIMIDINES
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
RATS
RODENTS
SULFUR 35
SULFUR ISOTOPES
TRACER TECHNIQUES
USES
VERTEBRATES