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Isolation and partial chemical characterization of a 64,000-dalton glycoprotein of human cytomegalovirus

Journal Article · · J. Virol.; (United States)
OSTI ID:5878592
; ; ;
A guanidinium chloride extract of (/sup 3/H)glucosamine- and (/sup 35/S)methionine-labeled virions plus dense bodies of human cytomegalovirus (Towne) was separated by reverse-phase high-pressure liquid chromatography. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the eluate revealed the major peak to be a glycoprotein with a relative mass of 64,000. This glycoprotein (HCMVgp64) was characterized by amino acid analysis and a high-pressure liquid chromatographic map of its tryptic peptides.
Research Organization:
City of Hope Research Inst., Duarte, CA
OSTI ID:
5878592
Journal Information:
J. Virol.; (United States), Journal Name: J. Virol.; (United States) Vol. 49:1; ISSN JOVIA
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
550701 -- Microbiology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINES
AMINO ACID SEQUENCE
AMINO ACIDS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOHYDRATES
CARBOXYLIC ACIDS
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
DRUGS
EMISSION SPECTROSCOPY
EVEN-ODD NUCLEI
FLUORESCENCE SPECTROSCOPY
GLUCOPROTEINS
GLUCOSAMINE
HEXOSAMINES
HEXOSES
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
LIPOTROPIC FACTORS
LIQUID COLUMN CHROMATOGRAPHY
MAMMALS
MAN
METHIONINE
MICROORGANISMS
MOLECULAR STRUCTURE
MOLECULAR WEIGHT
MONOSACCHARIDES
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PARASITES
PRIMATES
PROTEINS
RADIOISOTOPES
SACCHARIDES
SEPARATION PROCESSES
SPECTROSCOPY
SULFUR 35
SULFUR ISOTOPES
TRITIUM COMPOUNDS
VERTEBRATES
VIRUSES