Two-dimensional polyacrylamide gel analysis of Plodia interpunctella granulosis virus
The structural polypeptides of purified Plodia interpunctella granulosis virus were analyzed by three different two-dimensional gel systems. Isoelectric focusing followed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis allowed resolution of 53 acidic polypeptides in the enveloped nucleocapsid of the virus ranging in molecular weight from 97,300 to 8000. Nine of these polypeptides were shown to be glycoproteins by the technique of radiolabeled lectin blotting. Separation of the granulin in this system allowed resolution of five species, all of which have identical tryptic peptide maps. This matrix protein was demonstrated to be a phosphoglycoprotein by radiolabeled lectin blotting and acid phosphatase dephosphorylation. Nonequilibrium pH gel electrophoresis followed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis allowed resolution of the major basic protein of the virus, VP12, from a more acidic protein of the same molecular weight. Tryptic peptide analysis demonstrated that these two proteins were indeed different and acid urea gels followed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis allowed localization of the acidic protein to the envelope and the basic protein to the nucleocapsid of the virus. Finally, probing of the separated envelope nucleocapsid proteins in both the isoelectric focusing and nonequilibrium pH gel electrophoresis two-dimensional systems after transfer to nitrocellulose with iodinated, purified viral proteins allowed further insight into reactions which may be important in the maintenance of the virion structure.
- Research Organization:
- Kansas State Univ., Manhattan
- OSTI ID:
- 7118681
- Journal Information:
- J. Virol.; (United States), Vol. 60:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
POLYPEPTIDES
AUTORADIOGRAPHY
MOLECULAR STRUCTURE
VIRUSES
MOLECULAR BIOLOGY
ELECTROPHORESIS
GLUCOPROTEINS
IODINE 125
MOLECULAR WEIGHT
PH VALUE
VIRAL DISEASES
BETA DECAY RADIOISOTOPES
CARBOHYDRATES
DAYS LIVING RADIOISOTOPES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
INFECTIOUS DISEASES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPES
MICROORGANISMS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PARASITES
PEPTIDES
PROTEINS
RADIOISOTOPES
SACCHARIDES
550701* - Microbiology- Tracer Techniques
550201 - Biochemistry- Tracer Techniques