Proteins of hepatitis B surface antigen. [Polypeptide content]

Journal Article · Sat Jan 01 04:00:00 EST 1977 · J. Virol.; (United States)

OSTI ID:7317148

Shin, J W.K.; Gerin, J L

Purified 22-nm forms of hepatitis B surface antigen (HB/sub s/Ag) representing the three major antigenic subtypes (adw, ayw, and adr) were analyzed for their constituent polypeptides by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. No consistent difference in either the number or relative distributions of the polypeptides was observed for the various subtypes. Seven polypeptides were designated as P-1 through P-7 in order of their decreasing mobilities. By comparison with protein standards, their molecular weights were estimated as 23, 29.5, 36, 41.5, 53.5, 72, and 97 thousand. The P-1 and P-2 components represented the major polypeptides; P-2 and P-5 might be glycoproteins, based on their reaction with periodic acid-Shiff reagent. Each polypeptide contains cysteine residues. HB/sub s/Ag was radiolabeled with /sup 3/H or /sup 14/C by reductive methylation or iodinated with /sup 125/I by the chloramine-T or lactoperoxidase procedures. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of labeled HB/sub s/Ag yielded patterns identical to those obtained with protein stain. Comparison of HB/sub s/Ag labeled by the chloramine-T and lactoperoxide procedures indicated that there was no distinction between internal or external components within the 22-nm structure.

• Research Organization: Oak Ridge National Lab., Rockville, MD
• OSTI ID: 7317148
• Journal Information: J. Virol.; (United States), Journal Name: J. Virol.; (United States) Vol. 21:1; ISSN JOVIA
• Country of Publication: United States
• Language: English

Related Subjects

551000* -- Physiological Systems
59 BASIC BIOLOGICAL SCIENCES
ANTIGENS
CHEMICAL COMPOSITION
DIGESTIVE SYSTEM DISEASES
DISEASES
HEPATITIS
INFECTIOUS DISEASES
ORGANIC COMPOUNDS
PEPTIDES
POLYPEPTIDES
PROTEINS