Immunochemistry and polypeptide composition of hepatitis B core antigen (HB/sub c/ Ag). [/sup 125/I tracer techniques]

Journal Article · Fri Apr 01 04:00:00 EST 1977 · J. Immunol.; (United States)

OSTI ID:7107073

Budkowska, A; Shih, J W.K.; Gerin, J L

Core particles were isolated from a nuclear extract of a hepatitis B-infected liver, labeled with /sup 125/I by using chloramine-T and further purified by rate zonal sedimentation on sucrose gradients. Iodinated HB/sub c/ Ag was used as a ligand in a sensitive double-antibody radioimmunoprecipitation (RIP) assay for antibody to HB/sub c/ Ag. The specificity of the RIP reaction was evaluated using defined anti-HB/sub c/ sera and paired sera from six well-documented cases of hepatitis B infection. The polypeptide composition of the iodinated antigen was examined by SDS-polyacrylamide gel electrophoresis of solubilized complexes of /sup 125/I-HB/sub c/ Ag and anti-HB/sub c/. Two major polypeptides with apparent m.w. of 17,000 and 35,000 daltons were observed and designated as cP-1 and cP-2, respectively.

• Research Organization: Oak Ridge National Lab., Rockville, MD
• OSTI ID: 7107073
• Journal Information: J. Immunol.; (United States), Journal Name: J. Immunol.; (United States) Vol. 118:4; ISSN JOIMA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
AMINES
ANTIGENS
BETA DECAY RADIOISOTOPES
BIOCHEMISTRY
BODY
CHEMICAL COMPOSITION
CHEMISTRY
CHLORAMINES
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DIGESTIVE SYSTEM DISEASES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
ELECTROPHORESIS
GLANDS
HEPATITIS
IMMUNE REACTIONS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LIGANDS
LIVER
MOLECULAR WEIGHT
NUCLEI
ODD-EVEN NUCLEI
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANS
PARTICLES
PEPTIDES
POLYPEPTIDES
PROTEINS
RADIOISOTOPES
SEDIMENTATION
TRACER TECHNIQUES