Detection of glycoproteins in the Acanthamoeba plasma membrane

Journal Article · Tue Nov 01 00:00:00 EST 1988 · Experimental Cell Research; (United States)

DOI:https://doi.org/10.1016/0014-4827(88)90364-3· OSTI ID:5393294

Paatero, G I.L. ^[1]; Gahmberg, C G ^[2]

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1. Abo Akademi (Finland)
2. Univ. of Helsinki (Finland)

In the present study the authors have shown that glycoproteins are present in the plasma membrane of Acanthamoeba castellanii by utilizing different radioactive labeling techniques. Plasma membrane proteins in the amoeba were iodinated by {sup 125}I-lactoperoxidase labeling and the solubilized radiolabeled glycoproteins were separated by lectin-Sepharose affinity chromatography followed by polyacrylamide gel electrophoresis. The periodate/NaB{sup 3}H{sub 4} and galactose oxidase/NaB{sup 3}H{sub 4} labeling techniques were used for labeling of surface carbohydrates in the amoeba. Several surface-labeled glycoproteins were observed in addition to a diffusely labeled region with M{sub r} of 55,000-75,000 seen on electrophoresis, which could represent glycolipids. The presence of glycoproteins in the plasma membrane of Acanthamoeba castellanii was confirmed by metabolic labeling with ({sup 35}S)methionine followed by lectin-Sepharose affinity chromatography and polyacrylamide gel electrophoresis.

• OSTI ID: 5393294
• Journal Information: Experimental Cell Research; (United States), Vol. 179:1; ISSN 0014-4827
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CELL MEMBRANES
MOLECULAR STRUCTURE
GLYCOPROTEINS
BIOCHEMISTRY
LABELLED COMPOUNDS
UPTAKE
AMOEBA
CARBOHYDRATES
ELECTROPHORESIS
IODINE 125
METHIONINE
PEROXIDASES
SULFUR 35
TRITIUM COMPOUNDS
AMINO ACIDS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CHEMISTRY
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
EVEN-ODD NUCLEI
HYDROGEN COMPOUNDS
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
INVERTEBRATES
IODINE ISOTOPES
ISOTOPES
LIGHT NUCLEI
LIPOTROPIC FACTORS
MEMBRANES
MICROORGANISMS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
OXIDOREDUCTASES
PROTEINS
PROTOZOA
RADIOISOTOPES
SARCODINA
SULFUR ISOTOPES
550301* - Cytology- Tracer Techniques