Characterization of the O- and N-linked oligosaccharides in glycoproteins synthesized by Schistosoma mansoni

Thesis/Dissertation · Thu Jan 01 04:00:00 EST 1987

OSTI ID:6292351

Nyame, A K

The structures of the O- and N-linked oligosaccharides in glycoproteins synthesized by larval and adult schistosomes of Schistosoma mansoni have been investigated. Mechanically transformed schistosomula or adult schistosomes were incubated in media containing either (/sup 3/H)mannose, (/sup 3/H)glucosamine or (/sup 3/H)galactose for 48 and 24 hr, respectively, to radiolabel metabolically the oligosaccharide moieties of newly synthesized glycoproteins. Analyses of the radiolabeled glycoproteins by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS/PAGE) and fluorography demonstrated that numerous glycoproteins from 48-hr old schistosomula and adult schistosomes were labeled by both the (/sup 3/H)mannose and (/sup 3/H)glucosamine precursors. The (/sup 3/H)galactose precursor was incorporated into numerous glycoproteins in adult schistosomes; however, few, if any, glycoproteins in schistosomula were labeled by this radioactive sugar precursor.

• Research Organization: Georgia Univ., Athens (USA)
• OSTI ID: 6292351
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADULTS
AGE DEPENDENCE
AGE GROUPS
ALDEHYDES
AMINES
BIOSYNTHESIS
CARBOHYDRATES
ELECTROPHORESIS
GALACTOSE
GLUCOPROTEINS
GLUCOSAMINE
GLYCOPROTEINS
HELMINTHS
HEXOSAMINES
HEXOSES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LABELLING
LARVAE
MANNOSE
MOLECULAR STRUCTURE
MONOSACCHARIDES
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
PLATYHELMINTHS
PRECURSOR
PROTEINS
SACCHARIDES
SCHISTOSOMA
SYNTHESIS
TRACER TECHNIQUES
TREMATODES
TRITIUM COMPOUNDS