Characterization of the N-linked oligosaccharides in glycoproteins synthesized by mouse teratocarcinoma cells
Thesis/Dissertation
·
OSTI ID:7168276
The structures of the N-linked oligosaccharides in glycoproteins synthesized by mouse teratocarcinoma F9 cells and retinoic acid differentiated F9 cells have been investigated. F9 cells and retinoic acid differentiated F9 (RA/F9) cells were incubated in media containing either (2-{sup 3}H)mannose, (6-{sup 3}H) glucosamine for 24 hr to radiolabel metabolically the oligosaccharide moieties of newly synthesized glycoproteins. Analyses of the radiolabeled glycoproteins by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS/PAGE) and fluorography demonstrated that numerous glycoproteins from both cells were labeled by all three radioactive sugar precursors. Oligosaccharides were prepared from total-radiolabeled glycoproteins and analyzed by a variety of techniques. The transferrin receptor has been purified from both F9 and RA/F9 cells. The structures of N-linked oligosaccharides in the receptor were high mannose and complex-type.
- Research Organization:
- Georgia Univ., Athens, GA (USA)
- OSTI ID:
- 7168276
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
AMINES
ANIMAL CELLS
ANIMALS
CARBOHYDRATES
CARBOXYLIC ACID ESTERS
CHEMICAL COMPOSITION
ELECTROPHORESIS
ESTERS
GALACTOSE
GLOBULINS
GLOBULINS-BETA
GLUCOSAMINE
GLYCOPROTEINS
HEXOSAMINES
HEXOSES
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
MAMMALS
MEMBRANE PROTEINS
METALLOPROTEINS
MICE
MOLECULAR STRUCTURE
MONOSACCHARIDES
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
PROTEINS
RECEPTORS
RETINOIC ACID
RODENTS
SACCHARIDES
TRACER TECHNIQUES
TRANSFERRIN
TRITIUM COMPOUNDS
TUMOR CELLS
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
AMINES
ANIMAL CELLS
ANIMALS
CARBOHYDRATES
CARBOXYLIC ACID ESTERS
CHEMICAL COMPOSITION
ELECTROPHORESIS
ESTERS
GALACTOSE
GLOBULINS
GLOBULINS-BETA
GLUCOSAMINE
GLYCOPROTEINS
HEXOSAMINES
HEXOSES
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
MAMMALS
MEMBRANE PROTEINS
METALLOPROTEINS
MICE
MOLECULAR STRUCTURE
MONOSACCHARIDES
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
PROTEINS
RECEPTORS
RETINOIC ACID
RODENTS
SACCHARIDES
TRACER TECHNIQUES
TRANSFERRIN
TRITIUM COMPOUNDS
TUMOR CELLS
VERTEBRATES